생애초기 저산소증으로 인한 신경행동기형

Abstract

Background : Neurobehavioral teratology is a term used for the postnatal effects on behavior of prenatal exposure to drug or to specific environment. Perinatal hypoxia is a major risk factor for development of behavioral abnormalities, such as cerebral palsy, mental retardation and learning disability. The objective of this study is to investigate the effects of neonatal hypoxic stress. Methods : Sprague-Dawely rats were ground by hypoxia and/or 5-HT receptor antagonist treatment. Exposure to 100% N2 gas was done in postnatal day (PND) 2 for 12minutes. NAN-190 HBr or ketanserin tartrate or both were injection interaperitionelly 30 minutes before exposure to hypoxic environment. Rats were weighed periodically and examined the eye opening. Wire maneuver test was done on PND 22. Between PND 40-55 and PND 63-84, explorative behavior test and Rota-Rod test were measured by High Performance Liquid Chromatography- Electrochemical Detection(HPLC ECD) system. Receptor binding assay was done using 8-OH-DPAT and ketanserin than 5-HT receptor antagonist or hypoxia alone. There were no difference in weight gain, eye opening, and the result of wire maneuver test among each groups. In explorative behavior test. NAN+N2 group in male and NAN group in female showed markedly increased activities. In Rota-Rod test. NAN and NAN+N2 groups in both male and female and their metabolities, When measured in PND 100 according to the brain sites. There were no difference in the brain sites. There were no differences in pKd of 5-HT receptors measured on PND 100. But Bmax of 5-HT1A receptor were low in N2 NAN and NAN+ N2 groups. NAN period causes long-lasting neurobehavioral changes with neurochemical abnormalities, and 5-HT receptor activity has a role in that mechanism.