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Solution structures of the melanocyte-stimulating hormones by two-dimensional NMR spectroscopy and dynamical simulated-annealing calculations

Authors
 Jung‐Hoon Lee  ;  Sung‐Kil Lim  ;  Sung‐Ho Huh  ;  Donghan Lee  ;  Weontae Lee 
Citation
 EUROPEAN JOURNAL OF BIOCHEMISTRY, Vol.257(1) : 31-40, 1998 
Journal Title
 EUROPEAN JOURNAL OF BIOCHEMISTRY 
ISSN
 0014-2956 
Issue Date
1998
MeSH
Amino Acid Sequence ; Magnetic Resonance Spectroscopy ; Melanocyte-Stimulating Hormones/chemistry* ; Models, Molecular ; Protein Structure, Secondary
Abstract
Melanocortins, which are involved in melanocyte pigmentation control and glucocorticoid stimulation, have functional roles in various physiological mechanisms and have been shown to participate in higher cortical functions. Recently, it has also been reported that melanocyte-stimulating hormone (MSH) and melanocortin 4 receptor (MC4R) are the key components of the hypothalamic response to obesity. The solution structures of both melanocyte-stimulating hormone alpha-MSH (Ac-Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys-Pro-Val-NH2) and its analog alpha-MSH-ND (Ac-Ahx-Asp-His-DPhe-Arg-Trp-Lys-NH2) (Ahx, 2-aminohexanoic acid) have been determined by two-dimensional NMR spectroscopy and simulated-annealing calculations. The NMR data revealed that alpha-MSH forms a hairpin loop conformation which includes conserved message sequences, whereas alpha-MSH-ND prefers a type I beta-turn comprising residues of Asp2-His3-DPhe4-Arg5. Final simulated-annealing structures of both alpha-MSH-ND and alpha-MSH peptides converged with rmsd of 0.07 nm for alpha-MSH-ND and 0.1 nm for alpha-MSH between backbone atoms, respectively. This result will provide the structural bases of melanocortin functions as well as valuable information for structure-based drug design involving the regulation of obesity and feeding.
Full Text
https://febs.onlinelibrary.wiley.com/doi/abs/10.1046/j.1432-1327.1998.2570031.x
DOI
10.1046/j.1432-1327.1998.2570031.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Lim, Sung Kil(임승길)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177047
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