0 20

Cited 0 times in

Clinical significance of clonality in thyroid nodules

Authors
 H. KIM  ;  Z. PIAO  ;  C. PARK  ;  W. Y. CHUNG  ;  C. S. PARK 
Citation
 BRITISH JOURNAL OF SURGERY, Vol.85(8) : 1125-1128, 1998 
Journal Title
 BRITISH JOURNAL OF SURGERY 
ISSN
 0007-1323 
Issue Date
1998
MeSH
Adenoma/genetics* ; Adenoma/pathology ; Adenoma/surgery ; Adult ; Aged ; Carcinoma, Papillary, Follicular/genetics* ; Carcinoma, Papillary, Follicular/pathology ; Carcinoma, Papillary, Follicular/surgery ; Female ; Humans ; Middle Aged ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Prospective Studies ; Receptors, Androgen/genetics ; Thyroid Nodule/genetics* ; Thyroid Nodule/pathology ; Thyroid Nodule/surgery
Abstract
BACKGROUND: Many different neoplastic and hyperplastic thyroid diseases present with clinically apparent thyroid nodules. Clonality analysis indicates whether a nodule arises from the polyclonal proliferation of a group of cells or forms a clone from a genetically altered cell and thus provides objective information on the origin of the thyroid nodules. Clonality was studied in thyroid nodules using the polymerase chain reaction (PCR) assay in the X-linked human androgen receptor (HUMARA) gene by random X chromosome inactivation in women. METHODS: DNA samples were obtained from 28 nodules in 21 women. All nodules and non-tumour thyroid tissues were fractioned selectively under a cryostat. Genomic DNA was isolated and digested with HhaI. PCR amplification of the HUMARA locus was performed using PCR mixtures containing [alpha-32P]2'-deoxycytidine 5'-triphosphate. The PCR products were analysed by denaturing gel electrophoresis. RESULTS: The HUMARA alleles were heterogeneous in 18 of 21 patients. Among the 23 nodules from 18 patients, all of the eight papillary thyroid carcinomas were monoclonal. Two solitary nodules from follicular adenomas were monoclonal. Of the 13 follicular nodules from nodular goitres, ten were polyclonal and three were monoclonal. The monoclonal follicular nodules were larger in size (3.5 versus 2.0 cm, P< 0.05) and had a tendency towards more cystic changes than polyclonal nodules. CONCLUSION: PCR-based clonality study of thyroid nodules may help to distinguish hyperplastic from neoplastic nodules.
Full Text
https://bjssjournals.onlinelibrary.wiley.com/doi/abs/10.1046/j.1365-2168.1998.00783.x
DOI
10.1046/j.1365-2168.1998.00783.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hogeun(김호근)
Park, Chan Il(박찬일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/176932
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links