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Indirect mitogenic effect of transforming growth factor-beta on cell proliferation of subconjunctival fibroblasts
DC Field | Value | Language |
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dc.contributor.author | 이형근 | - |
dc.date.accessioned | 2020-07-02T17:33:26Z | - |
dc.date.available | 2020-07-02T17:33:26Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0146-0404 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/176889 | - |
dc.description.abstract | PURPOSE: To understand the mechanism of fibrosis after filtering surgery for glaucoma, the effect of transforming growth factor-beta (TGF-beta) was studied in subconjunctival fibroblasts (SCFs). TGF-beta, universal inhibitor of cell proliferation, stimulates the cell proliferation of fibroblasts. SCFs were evaluated for their production of TGF-beta and fibroblast growth factor 2 (FGF-2) to determine whether TGF-beta may be an indirect mitogen acting through the induction of an endogenous growth factor, or factors, that then acts as the direct mitogen in an autocrine manner. METHODS: Cell proliferation was determined either by counting cell numbers or by analyzing the incorporation of [3H]thymidine into DNA. The synthesis of TGF-beta and FGF-2 was analyzed by immunoprecipitation and immunoblotting. RESULTS: TGF-beta 1, TGF-beta 2, and TGF-beta 3 stimulated the cell proliferation of SCFs in a dose-dependent manner. The media conditioned by SCFs, which were subsequently activated by acid, stimulated cell proliferation of corneal stromal fibroblasts. When the acid-activated media conditioned by SCFs were immunoprecipitated, respectively, either with anti-TGF-beta 1 and TGF-beta 2 antibodies or with anti-TGF-beta 3 antibody, TGF-beta s, with an apparent molecular size of 25 kDa, were detected, whereas SCFs produced an 80-kDa latent form of TGF-beta 1. Interestingly, SCFs produced and secreted an 18-kDa extracellular isoform of FGF-2, the synthesis of which is further stimulated by TGF-beta 1 and TGF-beta 3, respectively, whereas the neutralizing antibody to FGF-2 and the FGF-2-specific antisense oligonucleotide primers inhibited the stimulatory activities of TGF-beta 1 in SCFs. CONCLUSIONS: These findings indicate that SCFs produce TGF-beta and FGF-2 and that FGF-2 seems to be the direct stimulator of TGF-beta-mediated cell proliferation in SCFs. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Association For Research In Vision And Ophthalmology (Arvo) | - |
dc.relation.isPartOf | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Count | - |
dc.subject.MESH | Cell Division/drug effects | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Conjunctiva/cytology | - |
dc.subject.MESH | Conjunctiva/drug effects* | - |
dc.subject.MESH | Conjunctiva/metabolism | - |
dc.subject.MESH | Culture Media, Conditioned | - |
dc.subject.MESH | DNA/biosynthesis | - |
dc.subject.MESH | DNA Replication | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Fibroblast Growth Factor 2/biosynthesis | - |
dc.subject.MESH | Fibroblast Growth Factor 2/genetics | - |
dc.subject.MESH | Fibroblasts/cytology | - |
dc.subject.MESH | Fibroblasts/drug effects | - |
dc.subject.MESH | Fibroblasts/metabolism | - |
dc.subject.MESH | Immunoblotting | - |
dc.subject.MESH | Mitogens/pharmacology* | - |
dc.subject.MESH | Oligonucleotides, Antisense/pharmacology | - |
dc.subject.MESH | Precipitin Tests | - |
dc.subject.MESH | Rabbits | - |
dc.subject.MESH | Transforming Growth Factor beta/biosynthesis | - |
dc.subject.MESH | Transforming Growth Factor beta/pharmacology* | - |
dc.title | Indirect mitogenic effect of transforming growth factor-beta on cell proliferation of subconjunctival fibroblasts | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학교실) | - |
dc.contributor.googleauthor | EunDuck P. Kay | - |
dc.contributor.googleauthor | Hyung Keun Lee | - |
dc.contributor.googleauthor | Kee Soon Park | - |
dc.contributor.googleauthor | Sung Chul Lee | - |
dc.contributor.localId | A03303 | - |
dc.relation.journalcode | J01187 | - |
dc.identifier.eissn | 1552-5783 | - |
dc.identifier.pmid | 9501857 | - |
dc.contributor.alternativeName | Lee, Hyung Keun | - |
dc.contributor.affiliatedAuthor | 이형근 | - |
dc.citation.volume | 39 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 481 | - |
dc.citation.endPage | 486 | - |
dc.identifier.bibliographicCitation | INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.39(3) : 481-486, 1998 | - |
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