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계대배양된 코점막 상피세포에서 분비상피세포로의 분화

Other Titles
 Secretory Differentiation of Serially Passaged Normal Human Nasal Epithelial (NHNE) Cells 
Authors
 윤주헌  ;  김경수  ;  김성식  ;  이성민  ;  이주환  ;  이정권 
Citation
 Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.41(8) : 1008-1014, 1998 
Journal Title
 Korean Journal of Otolaryngology (대한이비인후과학회지) 
ISSN
 1225-035X 
Issue Date
1998
Abstract
Background and Objectives:The purpose of this study was to subculture normal human nasal epithelial (NHNE)cells without compromising their ability to differentiate into secretory and ciliated cells and to study the effect of retinoic acid (RA)on mucous and serous secretions in each passaged cells. Materials and Method:Freshly isolated nasal epithelial cells from normal inferior turbinates were subcultured repeatedly in serum-free medium on plastic tissue culture dishes. The subcultured cells were tested after every passage for secretory differentiation in air-liquid interface (ALI) cultures. The apical secretion of cultured NHNE cells was characterized by immunoblotting and Western blotting. Results:Cultured NHNE cells secreted mucin and lysozyme. RA was essential for mucociliary and secretory differentiation. The epithelium became squamous and mucin secretion decreased when RA was deleted from the culture media. Cells from passage 1(P-1) through passage-2 (P-2) remained competent to differentiate into mucous and squamous cells when grown in air-liquid interface culture. Conclusion:P-2 NHNE cell cultures retained many important features of normal epithelium and were suitable for conducting many studies of upper airway cell biology with an expanded cell pool.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Su(김경수) ORCID logo https://orcid.org/0000-0003-1460-0640
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/176881
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