메밀의 재조합항원 제조

Abstract

Purpose : Buckwheat is one of the most common food allegen in Korea and frequently elicit severe allergic reactions. However, up to now, only few reports on buckwheat allergens have been reported. The purpose of this study was to isolate mRNA for the production of recombinant buckwheat allergens. Methods : After the isolation of mRNA from ripening buckwheat seeds, in vitro translation was performed. The protein patterns of in vitro translate products were identified using SDS-PAGE. Results : A_260/A_260 ratio of total RNA was 1.9, and mRNA was 2.0. In vitro translate products showed lowered molecular buckwheat proteins such as 1, 3, and l4kD, while other high molecular weight protein of buckwheat seed were not shown in SDS-PAGE. Conclusion : mRNA of buckwheat was purified only from ripening seeds. The composition of mRNA was different according to the ripening periods. It is believed that this finding can give a clue to the basic research of buckwheat allergen.