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Isolation of a Partial Human CBNA Encoding a Factor Binding to the Perfect Palindrome of Enhancer A of HLA Class I Promoters Homologous to NF-κB2
DC Field | Value | Language |
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dc.contributor.author | 김세종 | - |
dc.contributor.author | 박전한 | - |
dc.date.accessioned | 2020-07-02T17:26:53Z | - |
dc.date.available | 2020-07-02T17:26:53Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0253-3162 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/176817 | - |
dc.description.abstract | Genes encoding sequence-specific DNA binding proteins have been isolated by screening cDNA libraries constructed in rgt11 expression vector with recognition site DNAs. We isolated a rgt11 recombinant human cDNA clone, designated to C2, using a DNA probe consisted of heptamer of the perfect palindrome (PP; GGGGATTCCCC) of enhancer A (Enh A) of HLA dass I promoter. Sequencing analysis showed that this clone contained a partial cDNA homologous to NF-kB2. Lysogenic E. coli containing the C2 was generated and crude cell extract was prepared. Immunoblot using anti-B-galactosidase antibody showed that this lysogenic E. coli expressed B-galactosidase fusion protein. Electrophoretic mobility shift assay (EMSA) and DNase I footprinting assay were done using crude cell extract and their patterns were compared with nuclear protein extracted from an EBV transformed B lymphoblastoid cell line (BLCL). EMSA showed that crude cell extract prepared from E. coli lysogen speci5cally bound to the PP of Enh A region of HLA class I gene. DNase I footprinting assay showed that the binding sequence of this recombinant B-galactosidase fusion protein was identical to that of nuclear protein extracted from a BLCL. Our data indicate that a Agt11 recombinant cDNA clone was isolated from a human cDNA library using the PP of Enh A of the HLA class I promoter and this clone encoded a B-galactosidase fusion protein capable of binding to the PP and belongs to a NF-xB subunit. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | Korean | - |
dc.publisher | Korean Society for Microbiology | - |
dc.relation.isPartOf | Journal of the Korean Society for Microbiology (대한미생물학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Isolation of a Partial Human CBNA Encoding a Factor Binding to the Perfect Palindrome of Enhancer A of HLA Class I Promoters Homologous to NF-κB2 | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | Jeon Han Park | - |
dc.contributor.googleauthor | Tae Jin Lee | - |
dc.contributor.googleauthor | Se Jong Kim | - |
dc.contributor.localId | A00603 | - |
dc.contributor.localId | A01641 | - |
dc.relation.journalcode | J03751 | - |
dc.identifier.url | http://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE01489910 | - |
dc.contributor.alternativeName | Kim, Se Jong | - |
dc.contributor.affiliatedAuthor | 김세종 | - |
dc.contributor.affiliatedAuthor | 박전한 | - |
dc.citation.volume | 33 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 295 | - |
dc.citation.endPage | 306 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Society for Microbiology (대한미생물학회지), Vol.33(3) : 295-306, 1998 | - |
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