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Isolation of a Partial Human CBNA Encoding a Factor Binding to the Perfect Palindrome of Enhancer A of HLA Class I Promoters Homologous to NF-κB2

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dc.contributor.author김세종-
dc.contributor.author박전한-
dc.date.accessioned2020-07-02T17:26:53Z-
dc.date.available2020-07-02T17:26:53Z-
dc.date.issued1998-
dc.identifier.issn0253-3162-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/176817-
dc.description.abstractGenes encoding sequence-specific DNA binding proteins have been isolated by screening cDNA libraries constructed in rgt11 expression vector with recognition site DNAs. We isolated a rgt11 recombinant human cDNA clone, designated to C2, using a DNA probe consisted of heptamer of the perfect palindrome (PP; GGGGATTCCCC) of enhancer A (Enh A) of HLA dass I promoter. Sequencing analysis showed that this clone contained a partial cDNA homologous to NF-kB2. Lysogenic E. coli containing the C2 was generated and crude cell extract was prepared. Immunoblot using anti-B-galactosidase antibody showed that this lysogenic E. coli expressed B-galactosidase fusion protein. Electrophoretic mobility shift assay (EMSA) and DNase I footprinting assay were done using crude cell extract and their patterns were compared with nuclear protein extracted from an EBV transformed B lymphoblastoid cell line (BLCL). EMSA showed that crude cell extract prepared from E. coli lysogen speci5cally bound to the PP of Enh A region of HLA class I gene. DNase I footprinting assay showed that the binding sequence of this recombinant B-galactosidase fusion protein was identical to that of nuclear protein extracted from a BLCL. Our data indicate that a Agt11 recombinant cDNA clone was isolated from a human cDNA library using the PP of Enh A of the HLA class I promoter and this clone encoded a B-galactosidase fusion protein capable of binding to the PP and belongs to a NF-xB subunit.-
dc.description.statementOfResponsibilityrestriction-
dc.languageKorean-
dc.publisherKorean Society for Microbiology-
dc.relation.isPartOfJournal of the Korean Society for Microbiology (대한미생물학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleIsolation of a Partial Human CBNA Encoding a Factor Binding to the Perfect Palindrome of Enhancer A of HLA Class I Promoters Homologous to NF-κB2-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학교실)-
dc.contributor.googleauthorJeon Han Park-
dc.contributor.googleauthorTae Jin Lee-
dc.contributor.googleauthorSe Jong Kim-
dc.contributor.localIdA00603-
dc.contributor.localIdA01641-
dc.relation.journalcodeJ03751-
dc.identifier.urlhttp://www.dbpia.co.kr/journal/articleDetail?nodeId=NODE01489910-
dc.contributor.alternativeNameKim, Se Jong-
dc.contributor.affiliatedAuthor김세종-
dc.contributor.affiliatedAuthor박전한-
dc.citation.volume33-
dc.citation.number3-
dc.citation.startPage295-
dc.citation.endPage306-
dc.identifier.bibliographicCitationJournal of the Korean Society for Microbiology (대한미생물학회지), Vol.33(3) : 295-306, 1998-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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