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ABO 아형에서 ABO 유전자형 검사의 적용

Other Titles
 Application of ABO genotyping determination of ABO subgroups 
Authors
 김문정  ;  신정원  ;  김영환  ;  김현옥  ;  조성란  ;  김휘준 
Citation
 Korean Journal of Blood Transfusion (대한수혈학회지), Vol.9(2) : 209-217, 1998 
Journal Title
 Korean Journal of Blood Transfusion (대한수혈학회지) 
ISSN
 1226-9336 
Issue Date
1998
Abstract
Background : The knowledge about the nucleotides sequence of 9th chromosome that regulates the phenotype of ABO blood group has made the ABO genotyping possible. Since the genotyping can be done with only a small amount of DNA sample, it was primarily applied to the field of forensic medicine. When applied to the blood bank, it is useful in the resolution for ABO discrepancies between the cell and serum typing and determination of A and B subgroups. Rapid ABO genotyping using the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method and its value in determination of ABO subgroups is presented. Methods : ABO genotyping was performed in seven patients and three families, seven were the cases of ABO discrepancies in routine ABO grouping and three families were for the confirmation of the ABO group. To identify the 261th nucleotide, a 252 bp PCR amplifed fragment was amplified by PCR and digested with Kpn I. For 703th nucleotide, a 128 bp PCR amplified fragment was designed and digested with Alu I. To determine the ABO genotype, the patterns of digestion in DNA fragment were examined. Results : Among the seven cases of ABO discrepancies, B3 and Ael were two cases each. Weakened B due to leukemia was the one, and the other two cases were cis-AB and Am. The three families for confirmation of the ABO group were acquired B due to infection one family, cis-AB two families. Conclusions : ABO genotyping is a rapid and reliable method that can be used in the case of ABO discrepancies and determination of ABO subgroups.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/176609
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