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말초혈액 조혈모세포 채집시기 예측인자로서 채집전 말초혈액의 백혈구수, 단핵구수 및 CD34+ 세포수의 유용성 평가

Other Titles
 Preapheresis WBC, mononuclear cell and CD34+ cell counts as predictors of optimal timing of peripheral blood stem cell collection 
 김문정  ;  박규은  ;  임현숙  ;  김현옥  ;  민유홍  ;  유철주  ;  남정현 
 Korean Journal of Blood Transfusion (대한수혈학회지), Vol.9(1) : 101-110, 1998 
Journal Title
 Korean Journal of Blood Transfusion (대한수혈학회지) 
Issue Date
Background : Peripheral blood stem cells (PBSC) transplantation has been widely used as a substitute of bone marrow transplantation in patients with hematologic malignancies and solid tumors. Because, PBSC harvest by serial daily apheresis procedure is expensive and time consuming, it is important to determine the best time to start the collection for reducing the number of apheresis procedure. We analyzed our experiences of PBSC collections and evaluated the preapheresis hematologic parameters that may predict the PBSC yields. Methods : One hundred seventy six PBSC harvests from seventy cancer patients (median age : 32 yrs; fourty five males and twenty five females) were performed using our large volume leukapheresi. protocol (total blood volume processed : over three total blood volume) after chemotherapy and infusion of G-CSF. Peripheral blood obtained immediately before the start of apheresis was analyzed for total WBC, mononuclear cell (MNC), and CD34+ cell counts. Total WBC, MNC, and CD34+ cell count were performed on selected samples of PBSC from each patient before freezing for determining the PBSC yields. Linear regression analysis was performed on logarithmized data whether preapheresis WBC, MNC, and CD34+ cell counts on the day of harvest in the peripheral blood might correlate well with the PBSC yield, respectively. Results : With the use of linear regression analysis, preapheresis WBC counts and MNC counts were not correlated significantly with the CD34+ cell yield in PBSC harvests (WBC/μL in PB vs. CD34+ cell/kg in harvests, r=0.35, p=0.10; MNC/μL in PB vs. CD34+ cells/kg in harvests, r=0.4 2, p=0.07). But the CD34+ cell count (CD34+ cells/ μL in peripheral blood) correlated most closely with the progenitor cell yield in the corresponding leukapheresis product (CD34+ cell/kg body weight, r=0.75, p<0.001). A number of 20 circulating CD34+ cells/μL blood ensured 2.0×106 CD34+ cells/kg, that is known to be a threshold dose for rapid hematologic recovery, and the best time for the collection on the same day by a single leukapheresis in more than 85% cases. Conclusions : The number of CD34+ cells/μL blood allows a reliable prediction of the CD34+progenitor cell yield in subsequent leukapheresis procedure, while WBC and MNC counts did not predict the progenitor cell yield. A level of more than 20 CD34+ cells/μL indicates that the threshold quantity of 2.0×106 CD34+ cells/kg is likely to be obtained by a single leukapheresis processing 15∼20 liters of peripheral blood.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Ok(김현옥) ORCID logo https://orcid.org/0000-0002-4964-1963
Min, Yoo Hong(민유홍) ORCID logo https://orcid.org/0000-0001-8542-9583
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