Cited 24 times in
Effects of intravenous immunoglobulin on plasma interleukin-10 levels in Kawasaki disease
DC Field | Value | Language |
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dc.contributor.author | 이원영 | - |
dc.date.accessioned | 2020-07-02T16:58:49Z | - |
dc.date.available | 2020-07-02T16:58:49Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0165-2478 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/176501 | - |
dc.description.abstract | Kawasaki disease is an acute febrile disease in children and many inflammatory cytokines are known to be involved in its pathogenesis. Interleukin-10 (IL-10) is an anti-inflammatory cytokine and previous reports have shown blood IL-10 levels were elevated during the acute phase of Kawasaki disease. In this study, we endeavoured to clarify the effects of IVIG on plasma IL-10 levels and to verify by RT-PCR, using specific primers, whether viral IL-10 or human IL-10 was responsible for high plasma IL-10. T-cells and B-cells were separated using magnetic beads, and IL-10 mRNA expressions were tested in both cell types. The mean plasma IL-10 levels were significantly decreased from 125.037±111.161 pg/ml, before IVIG treatment, to 32.437±54.716 pg/ml, after IVIG treatment, in subjects with Kawasaki disease. The levels of patients before IVIG treatment were significantly higher than those of the acute febrile patients (mean 26.956±13.316 pg/ml) and the normal controls (mean 16.042±5.088 pg/ml). RT-PCR was performed using specific primers to distinguish whether viral IL-10 (BCRF-1) or human IL-10 was produced in Kawasaki disease. Viral IL-10 (BCRF-1) mRNA expression was not detected in any groups and only human IL-10 mRNA transcripts were detected in PBMCs of Kawasaki patients as well as in those of acute febrile patients and normal controls. Human IL-10 mRNA transcripts were detected in both CD3+ T-cells and CD19+ B-cells. Elevated IL-10 levels during the acute phase of Kawasaki disease decreased immediately after IVIG administration, coincidentally with rapid improvement of inflammatory symptoms. Elevated plasma IL-10 in Kawasaki disease was transcripted from the human IL-10 gene and IL-10 mRNA expressions were detected in both T- and B-cells. Therefore, this study suggests that plasma IL-10 levels may be useful in the early identification and discrimination of the acute phase of Kawasaki disease from other febrile diseases. However, IVIG effects on IL-10 production still needs further investigation. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Elsevier/North-Holland Biomedical Press | - |
dc.relation.isPartOf | IMMUNOLOGY LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Effects of intravenous immunoglobulin on plasma interleukin-10 levels in Kawasaki disease | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | GeunWoong Noh | - |
dc.contributor.googleauthor | WooGill Lee | - |
dc.contributor.googleauthor | Wonyoung Lee | - |
dc.contributor.googleauthor | Kiyoung Lee | - |
dc.identifier.doi | 10.1016/S0165-2478(98)00016-9 | - |
dc.contributor.localId | A03003 | - |
dc.relation.journalcode | J01038 | - |
dc.identifier.eissn | 1879-0542 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0165247898000169#! | - |
dc.contributor.alternativeName | Lee, Won Young | - |
dc.contributor.affiliatedAuthor | 이원영 | - |
dc.citation.volume | 62 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 19 | - |
dc.citation.endPage | 24 | - |
dc.identifier.bibliographicCitation | IMMUNOLOGY LETTERS, Vol.62(1) : 19-24, 1998 | - |
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