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독일바퀴 알레르겐의 특성규명에 관한 연구

Other Titles
 Identification and Characterization of German Cockroach Allergen 
Authors
 이기영  ;  정병주  ;  김규언  ;  류정우  ;  염혜영  ;  박중원  ;  홍천수  ;  이한일  ;  용태순 
Citation
 Pediatric Allergy and Respiratory Disease (소아알레르기 호흡기학회지), Vol.8(2) : 221-228, 1998 
Journal Title
 Pediatric Allergy and Respiratory Disease (소아알레르기 호흡기학회지) 
ISSN
 1225-679X 
Issue Date
1998
Abstract
Purpose : Cockroaches have been reported one of the major causes of allergic disease such as bronchial asthma and allergic rhinitis. About 10% of children and 20% of adult respiratory allergy patients have positive results of allergy skin tests to cockroach allergen. This finding suggests that cockroach is also important allergen in Korea. Among 4,000 species, German cockroach seems predominant species in the Korean peninsula. Identification and characterization of the major allergen is the first step in German cockroach allergen research. But, there have been controversies. It may depends upon different identifying methods. The aim of our study was to identify the major allergen in German cockroach using Westernblot. Methods : Crude German cockroach allergen extracts with or without protease inhibitor cocktail mixture were prepared, and protein patterns of these two extracts were compared using SDS-PAGE and Coomassie stain. Sera from 32 atopic asthmatic patients with positive allergy skin test against cockroach allergen were used for IgE immunoblot. Results : Coomassie stain after SDS-PAGE demonstrated some discordance between the differently prepared crude allergen extracts. In comparison with the allergen extract which abolished endogenous protease activity, the untreated extract revealed new 32, 55 and 78 kD protein bands and 41 kD protein band was disappeared. About 60% of sera with positive allergy skin test revealed IgE binding bands in Westernblot. Among them, 10, 21, 25, 36 and 52 kD protein band might seemed important German cockroach allergen. Conclusion : Finding above may suggest that endogenous protease could denature allergenic proteins in the process of crude cockroach allergen extract. The 10, 21, 25, 36, and 52 kD might be the important allergens in German cockroach.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyu Earn(김규언)
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Ree, Han Il(이한일)
Hong, Chein Soo(홍천수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/176366
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