Cited 8 times in
Therapeutic Potential of miR-21 Regulation by Human Peripheral Blood Derived-Small Extracellular Vesicles in Myocardial Infarction
DC Field | Value | Language |
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dc.contributor.author | 정보영 | - |
dc.contributor.author | 김효은 | - |
dc.date.accessioned | 2020-06-17T00:56:44Z | - |
dc.date.available | 2020-06-17T00:56:44Z | - |
dc.date.issued | 2020-04 | - |
dc.identifier.issn | 0143-5221 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/176192 | - |
dc.description.abstract | Small extracellular vesicles (sEVs) as natural membranous vesicles are on the frontiers of nanomedical research, due to their ability to deliver therapeutic molecules such as microRNAs (miRNAs). The miRNA-21 (miR-21) is thought to be involved in the initiation and development of myocardial infarction (MI). Here, we examined whether miR-21 regulation using human peripheral blood-derived sEVs (PB-sEVs) could serve as a potential therapeutic strategy for MI. First, we examined miR-21 levels in hypoxic conditions and validated the ability of PB-sEVs to serve as a potential delivery system for miRNAs. Further, bioinformatics analysis and luciferase assay were performed to identify target genes of miR-21 mechanistically. Among numerous target pathways, we focused on nitrogen metabolism, which remains relatively unexplored compared with other possible miR-21-mediated pathways; hence, we aimed to determine novel target genes of miR-21 related to nitrogen metabolism. In hypoxic conditions, the expression of miR-21 was significantly up-regulated and correlated with nitric oxide synthase 3 (NOS3) levels, which in turn influences cardiac function. The down-regulation of miR-21 expression by PB-sEVs loaded with anti-miR-21 significantly improved survival rates, consistent with the augmentation of cardiac function. However, the up-regulation of miR-21 expression by PB-sEVs loaded with miR-21 reversed these effects. Mechanistically, miR-21 targeted and down-regulated the mRNA and protein expression of striatin (STRN), which could regulate NOS3 expression. In conclusion, we identified a novel therapeutic strategy to improve cardiac function by regulating the expression of miR-21 with PB-sEVs as an miR-21 or anti-miR-21 delivery vehicle and confirmed the miR-21-associated nitrogen metabolic disorders in MI. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Portland Press | - |
dc.relation.isPartOf | CLINICAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Therapeutic Potential of miR-21 Regulation by Human Peripheral Blood Derived-Small Extracellular Vesicles in Myocardial Infarction | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Ji-Young Kang | - |
dc.contributor.googleauthor | Hyoeun Kim | - |
dc.contributor.googleauthor | Dasom Mun | - |
dc.contributor.googleauthor | Nuri Yun | - |
dc.contributor.googleauthor | Boyoung Joung | - |
dc.identifier.doi | 10.1042/CS20191077 | - |
dc.contributor.localId | A03609 | - |
dc.relation.journalcode | J00613 | - |
dc.identifier.eissn | 1470-8736 | - |
dc.identifier.pmid | 32297634 | - |
dc.identifier.url | https://portlandpress.com/clinsci/article-lookup/doi/10.1042/CS20191077 | - |
dc.subject.keyword | human peripheral blood | - |
dc.subject.keyword | miR-21 | - |
dc.subject.keyword | myocardial infarction | - |
dc.subject.keyword | small extracellular vesicles | - |
dc.contributor.alternativeName | Joung, Bo Young | - |
dc.contributor.affiliatedAuthor | 정보영 | - |
dc.citation.volume | 134 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 985 | - |
dc.citation.endPage | 999 | - |
dc.identifier.bibliographicCitation | CLINICAL SCIENCE, Vol.134(8) : 985-999, 2020-04 | - |
dc.identifier.rimsid | 67493 | - |
dc.type.rims | ART | - |
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