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O-GlcNAcylation of Light Chain Serine 12 Mediates Rituximab Production Doubled by Thiamet G

DC FieldValueLanguage
dc.contributor.author김주영-
dc.date.accessioned2020-06-17T00:45:15Z-
dc.date.available2020-06-17T00:45:15Z-
dc.date.issued2020-05-
dc.identifier.issn1615-7591-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/176105-
dc.description.abstractO-Glycosylation occurs in recombinant proteins produced by CHO cells, but this phenomenon has not been studied extensively. Here, we report that rituximab is an O-linked N-acetyl-glucosaminylated (O-GlcNAcylated) protein and the production of rituximab is increased by thiamet G, an inhibitor of O-GlcNAcase. The production of rituximab doubled with OGA inhibition and decreased with O-GlcNAc transferase inhibition. O-GlcNAc-specific antibody and metabolic labelling with azidO-GlcNAc confirmed the increased O-GlcNAcylation with thiamet G. Protein mass analysis revealed that serine 7, 12, and 14 of the rituximab light chain were O-GlcNAcylated. S12A mutation of the light chain decreased rituximab stability and failed to increase the production with thiamet G without any significant changes of mRNA level. Cytotoxicity and thermal stability assays confirmed that there were no differences in the biological and physical properties of rituximab produced by thiamet G treatment. Therefore, thiamet G treatment improves the production of rituximab without significantly altering its function.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherSpringer-Verlag-
dc.relation.isPartOfBIOPROCESS AND BIOSYSTEMS ENGINEERING-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleO-GlcNAcylation of Light Chain Serine 12 Mediates Rituximab Production Doubled by Thiamet G-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학교실)-
dc.contributor.googleauthorHye-Yeon Kim-
dc.contributor.googleauthorMinseong Park-
dc.contributor.googleauthorChoeun Kang-
dc.contributor.googleauthorWoon Heo-
dc.contributor.googleauthorSei Mee Yoon-
dc.contributor.googleauthorJinu Lee-
dc.contributor.googleauthorJoo Young Kim-
dc.identifier.doi10.1007/s00449-020-02282-z-
dc.contributor.localIdA00942-
dc.relation.journalcodeJ03803-
dc.identifier.eissn1615-7605-
dc.identifier.pmid31980903-
dc.identifier.urlhttps://link.springer.com/article/10.1007/s00449-020-02282-z-
dc.subject.keywordADCC-
dc.subject.keywordCDC-
dc.subject.keywordO-GlcNAc-
dc.subject.keywordProduction yield-
dc.subject.keywordRituximab-
dc.subject.keywordThermal stability-
dc.subject.keywordThiamet G-
dc.contributor.alternativeNameKim, Joo Young-
dc.contributor.affiliatedAuthor김주영-
dc.citation.volume43-
dc.citation.number5-
dc.citation.startPage863-
dc.citation.endPage875-
dc.identifier.bibliographicCitationBIOPROCESS AND BIOSYSTEMS ENGINEERING, Vol.43(5) : 863-875, 2020-05-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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