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O-GlcNAcylation of Light Chain Serine 12 Mediates Rituximab Production Doubled by Thiamet G
DC Field | Value | Language |
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dc.contributor.author | 김주영 | - |
dc.date.accessioned | 2020-06-17T00:45:15Z | - |
dc.date.available | 2020-06-17T00:45:15Z | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 1615-7591 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/176105 | - |
dc.description.abstract | O-Glycosylation occurs in recombinant proteins produced by CHO cells, but this phenomenon has not been studied extensively. Here, we report that rituximab is an O-linked N-acetyl-glucosaminylated (O-GlcNAcylated) protein and the production of rituximab is increased by thiamet G, an inhibitor of O-GlcNAcase. The production of rituximab doubled with OGA inhibition and decreased with O-GlcNAc transferase inhibition. O-GlcNAc-specific antibody and metabolic labelling with azidO-GlcNAc confirmed the increased O-GlcNAcylation with thiamet G. Protein mass analysis revealed that serine 7, 12, and 14 of the rituximab light chain were O-GlcNAcylated. S12A mutation of the light chain decreased rituximab stability and failed to increase the production with thiamet G without any significant changes of mRNA level. Cytotoxicity and thermal stability assays confirmed that there were no differences in the biological and physical properties of rituximab produced by thiamet G treatment. Therefore, thiamet G treatment improves the production of rituximab without significantly altering its function. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Springer-Verlag | - |
dc.relation.isPartOf | BIOPROCESS AND BIOSYSTEMS ENGINEERING | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | O-GlcNAcylation of Light Chain Serine 12 Mediates Rituximab Production Doubled by Thiamet G | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학교실) | - |
dc.contributor.googleauthor | Hye-Yeon Kim | - |
dc.contributor.googleauthor | Minseong Park | - |
dc.contributor.googleauthor | Choeun Kang | - |
dc.contributor.googleauthor | Woon Heo | - |
dc.contributor.googleauthor | Sei Mee Yoon | - |
dc.contributor.googleauthor | Jinu Lee | - |
dc.contributor.googleauthor | Joo Young Kim | - |
dc.identifier.doi | 10.1007/s00449-020-02282-z | - |
dc.contributor.localId | A00942 | - |
dc.relation.journalcode | J03803 | - |
dc.identifier.eissn | 1615-7605 | - |
dc.identifier.pmid | 31980903 | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s00449-020-02282-z | - |
dc.subject.keyword | ADCC | - |
dc.subject.keyword | CDC | - |
dc.subject.keyword | O-GlcNAc | - |
dc.subject.keyword | Production yield | - |
dc.subject.keyword | Rituximab | - |
dc.subject.keyword | Thermal stability | - |
dc.subject.keyword | Thiamet G | - |
dc.contributor.alternativeName | Kim, Joo Young | - |
dc.contributor.affiliatedAuthor | 김주영 | - |
dc.citation.volume | 43 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 863 | - |
dc.citation.endPage | 875 | - |
dc.identifier.bibliographicCitation | BIOPROCESS AND BIOSYSTEMS ENGINEERING, Vol.43(5) : 863-875, 2020-05 | - |
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