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A Modified Magnified Analysis of Proteome (MAP) Method for Super-Resolution Cell Imaging that Retains Fluorescence

Authors
 Jiwon Woo  ;  Jeong-Min Seo  ;  Mirae Lee  ;  Juyoung Kim  ;  Sol Min  ;  Sang-Tae Kim  ;  Seockmo Ku  ;  Jeong-Yoon Park 
Citation
 SCIENTIFIC REPORTS, Vol.10(1) : e4186, 2020 
Journal Title
 SCIENTIFIC REPORTS 
Issue Date
2020
Abstract
Biological systems consist of a variety of distinct cell types that form functional networks. Super-resolution imaging of individual cells is required for better understanding of these complex systems. Direct visualization of 3D subcellular and nano-scale structures in cells is helpful for the interpretation of biological interactions and system-level responses. Here we introduce a modified magnified analysis of proteome (MAP) method for cell super-resolution imaging (Cell-MAP) which preserves cell fluorescence. Cell-MAP expands cells more than four-fold while preserving their overall architecture and three-dimensional proteome organization after hydrogel embedding. In addition, Optimized-Cell-MAP completely preserves fluorescence and successfully allows for the observation of tagged small molecular probes containing peptides and microRNAs. Optimized-Cell-MAP further successfully applies to the study of structural characteristics and the identification of small molecules and organelles in mammalian cells. These results may give rise to many other applications related to the structural and molecular analysis of smaller assembled biological systems.
Files in This Item:
T202000883.pdf Download
DOI
10.1038/s41598-020-61156-2
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Park, Jeong Yoon(박정윤) ORCID logo https://orcid.org/0000-0002-3728-7784
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/175640
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