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Establishment of patient-derived three-dimensional organoid culture in renal cell carcinoma

Authors
 Joon Chae Na  ;  Jee-Hoon Kim  ;  Sook Young Kim  ;  Young-Ran Gu  ;  Dae-Young Jun  ;  Hyung Ho Lee  ;  Young Eun Yoon  ;  Kyung Hwa Choi  ;  Sung Joon Hong  ;  Woong Kyu Han 
Citation
 INVESTIGATIVE AND CLINICAL UROLOGY, Vol.61(2) : 216-223, 2020 
Journal Title
 INVESTIGATIVE AND CLINICAL UROLOGY 
ISSN
 2466-0493 
Issue Date
2020
Keywords
Carcinoma, renal cell ; Organoids ; Precision medicine
Abstract
Purpose: Renal cell carcinoma is a heterogeneous kidney cancer, and over 403,000 cases were reported worldwide in 2018. Current methods for studying renal cell carcinoma are limited to two-dimensional (2D) culture of primary cell lines and patient-derived xenograft models. Numerous studies have suggested that 2D culture poorly represents the diversity, heterogeneity, and drug-resistance of primary tumors. The time and cost associated with patient-derived xenograft models poses a realistic barrier to their clinical utility. As a biomimetic model, patient-derived three-dimensional (3D) organoid culture can overcome these disadvantages and bridge the gap between in vitro cell culture and in vivo patient-derived xenograft models. Here, we establish a patient-derived 3D organoid culture system for clear cell renal cell carcinoma and demonstrate the biomimetic characteristics of our model with respect to both primary kidney cancer and conventional 2D culture. Materials and Methods: Normal renal tissues and tumor tissues were collected from patients with clear cell renal cell carcinoma. The dissociated cells were cultured as conventional 2D culture and 3D organoid culture. The biomimetic characteristic of the two cultures were compared. Results: Compared with 2D culture, the 3D organoid cultures retained the characteristic lipid-rich, clear cell morphology of clear cell renal cell carcinoma. Carbonic anhydrase 9 and vimentin were validated as biomarkers of renal cell carcinoma. Expression of the two validated biomarkers was more enhanced in 3D organoid culture. Conclusions: Patient-derived 3D organoid culture retains the characteristics of renal cell carcinoma with respect to morphology and biomarker expression.
Files in This Item:
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DOI
10.4111/icu.2020.61.2.216
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers
Yonsei Authors
Na, Joon Chae(나준채) ORCID logo https://orcid.org/0000-0003-4449-8472
Yoon, Young Eun(윤영은)
Lee, Hyung Ho(이형호)
Han, Woong Kyu(한웅규) ORCID logo https://orcid.org/0000-0002-2527-4046
Hong, Sung Joon(홍성준) ORCID logo https://orcid.org/0000-0001-9869-065X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/175628
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