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Bile duct ligation of C57BL/6 mice as a model of hepatic encephalopathy

 Inja Cho  ;  Bon-Nyeo Koo  ;  Eun Hee Kam  ;  Su Kyoung Lee  ;  Hanseul Oh  ;  So Yeon Kim 
 Anesthesia and Pain Medicine, Vol.15(1) : 19-27, 2020 
Journal Title
Anesthesia and Pain Medicine
Issue Date
Bile duct ligation ; C57BL/6 mouse ; Hepatic encephalopathy ; Liver dysfunction ; Passive avoidance test
Background: Bile duct ligation (BDL) has been used for experimental research on hepatic
encephalopathy (HE) caused by chronic liver disease. However, little research has
been done on a BDL model in C57BL/6 mouse. Therefore, we evaluated the suitability
of a BDL model in C57BL/6 mouse for the study of HE and determined which behavioral
tests are appropriate for the identification of HE in this model.
Methods: Twelve to fourteen-week-old male C57BL/6 mice were randomly assigned to
either sham group or BDL group. Histological changes in liver were confirmed by hematoxylin/
eosin and Masson’s trichrome staining. Liver function alterations were detected
by alanine aminotransferase (ALT) and ammonia levels. To identify behavioral changes,
open field, elevated plus maze, novel object recognition, and passive avoidance tests
were performed.
Results: Inflammatory liver injury and fibrosis were observed 14 days after BDL. ALT
and ammonia levels were significantly higher in BDL group than in sham group. There
were no differences in general locomotor activity or anxiety between the groups. No difference
was observed between these two groups in the novel object recognition test, but
BDL group showed significant learning/memory impairment in the passive avoidance
test compared to sham group.
Conclusions: Fourteen days of BDL in 12–14-week-old male C57BL/6 mice is a clinically
relevant model for HE, as these mice have liver fibrosis with impaired liver function,
hyperammonemia, and learning/memory impairment. Passive avoidance can be used
as the major behavioral test in this model of HE.
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1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Koo, Bon-Nyeo(구본녀) ORCID logo https://orcid.org/0000-0002-3189-1673
Kim, So Yeon(김소연) ORCID logo https://orcid.org/0000-0001-5352-157X
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