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Detection of recurrent, rare, and novel gene fusions in patients with acute leukemia using next-generation sequencing approaches

 Borahm Kim  ;  Esl Kim  ;  Seung‐Tae Lee  ;  June‐Won Cheong  ;  Chuhl Joo Lyu  ;  Yoo Hong Min  ;  Jong Rak Choi 
 HEMATOLOGICAL ONCOLOGY, Vol.38(1) : 82-88, 2020 
Journal Title
Issue Date
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor/genetics* ; Child ; Child, Preschool ; Female ; Gene Fusion* ; Genomics/methods* ; High-Throughput Nucleotide Sequencing/methods* ; Humans ; Leukemia, Myeloid, Acute/genetics* ; Leukemia, Myeloid, Acute/pathology ; Male ; Middle Aged ; Oncogene Proteins, Fusion* ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics* ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/pathology ; Prognosis ; Recurrence ; Young Adult
RNA gene fusion detection ; acute leukemia ; next-generation sequencing
Identification of gene fusion is an essential part in the management of patients with acute leukemia, not only for diagnosis but also in predicting the treatment outcome and selecting appropriate treatment. Adopting next-generation sequencing (NGS) technology for identification of gene fusion in patients with acute leukemia can be a good alternative to conventional tests. In the present study, the NGS RNA fusion gene panel test was applied to diagnostic samples of patients with acute leukemia to identify fusion genes more efficiently. Among 134 patients with acute leukemia, 53 gene fusions were detected in 52 patients. In addition to the recurrent gene fusions specified in the WHO diagnostic criteria, 11 rare or novel gene fusions were identified. Of those, two were gene fusions associated with Philadelphia-like acute lymphoblastic leukemia (Ph-like ALL), two were novel gene fusions, three were gene fusions with novel partner genes, and six were rare gene fusions from previous reports. We confirmed the clinical utility of the NGS test in identifying clinically significant gene fusions such as gene fusions involving KMT2A that has a large number of partners. Notably, Ph-like ALL-associated gene fusions could be easily identified despite the wide variety of genes involved. The results from the present study may contribute toward a better understanding of the genomic landscape of acute leukemia as well as patient management.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Borahm(김보람) ORCID logo https://orcid.org/0000-0003-0923-7744
Min, Yoo Hong(민유홍) ORCID logo https://orcid.org/0000-0001-8542-9583
Lyu, Chuhl Joo(유철주) ORCID logo https://orcid.org/0000-0001-7124-7818
Lee, Seung-Tae(이승태) ORCID logo https://orcid.org/0000-0003-1047-1415
Cheong, June-Won(정준원) ORCID logo https://orcid.org/0000-0002-1744-0921
Choi, Jong Rak(최종락) ORCID logo https://orcid.org/0000-0002-0608-2989
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