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ΔNp63 to TAp63 expression ratio as a potential molecular marker for cervical cancer prognosis

 Sunyoung Park  ;  Suji Lee  ;  Jungho Kim  ;  Geehyuk Kim  ;  Kwang Hwa Park  ;  Tae Ue Kim  ;  Dawn Chung  ;  Hyeyoung Lee 
 PLOS ONE, Vol.14(4) : e0214867, 2019 
Journal Title
Issue Date
Biomarkers, Tumor/genetics* ; Carcinogenesis/genetics ; Cell Line, Tumor ; Cervix Uteri/metabolism ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Middle Aged ; Precancerous Conditions/genetics ; Precancerous Conditions/metabolism ; Precancerous Conditions/pathology ; Prognosis ; Protein Isoforms/genetics ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Squamous Intraepithelial Lesions of the Cervix/genetics ; Squamous Intraepithelial Lesions of the Cervix/metabolism ; Squamous Intraepithelial Lesions of the Cervix/pathology ; Transcription Factors/genetics* ; Tumor Suppressor Proteins/genetics* ; Uterine Cervical Neoplasms/genetics* ; Uterine Cervical Neoplasms/metabolism ; Uterine Cervical Neoplasms/pathology
p63 is a transcription factor p53 family. Two major isoforms of p63, TAp63 with transactivation (TA) domain and ΔNp63 with truncated TA domain, have been reported to play opposing roles either in tumor suppression or oncogenic function. Little is known about the association of these two isoforms of p63 in the carcinogenesis of cervical cancer. In this study, the mRNA expression levels of TAp63 and ΔNp63 in 40 normal, 30 low-grade squamous intraepithelial lesions (LSIL), 38 high-grade squamous intraepithelial lesions (HSIL), and 52 cervical cancer formalin-fixed paraffin-embedded tissues were examined using quantitative reverse transcription polymerase chain reaction (RT-qPCR). We analyzed the association between the ΔNp63 and ΔN/TAp63 mRNA expression ratio and clinicopathological parameters and compared disease-specific survival of each ΔNp63 mRNA expression and ΔN/TAp63 mRNA expression ratio. The ΔN/TAp63 mRNA expression ratio in cervical cancer showed higher sensitivity than the mRNA expression levels of ΔNp63 (52.0% vs 44.2%). The level of ΔN/TAp63 mRNA expression ratio in precancerous LSIL and HSIL was higher than in normal tissues (P = 0.01 and P = 0.003) and lower than in cervical cancer tissues (P = 0.03 and P = 0.02). Besides, the positive ΔN/TAp63 mRNA expression ratio was associated with bulky tumor size and high expression of Ki-67, the proliferation marker, in cervical cancer (P = 0.04 and P = 0.02). The cervical cancer patients with the positive ΔN/TAp63 mRNA expression ratio showed worse survival compared to those who with the negative expression ratio of ΔN/TAp63 (HR = 5.7, 95% CI: 1.6-19.9). In conclusion, the balance of TAp63 and ΔNp63 is closely related to the carcinogenesis of cervical cancer. The ΔN/TAp63 mRNA expression ratio could be useful as a diagnostic and prognostic marker of cervical cancer.
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1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Chung, Da Wn(정다은)
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