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In vitro & in vivo evaluation of anti-fibrotic effects of Rg3-enhanced extract and identification of the associated miRNAs in endometriosis

Other Titles
 자궁내막증에서 Rg3 강화 추출물의 항섬유화 효과에 대한 생체내, 외 평가 및 이와 연관된 miRNA 규명 
 College of Medicine (의과대학) 
 Dept. of Obstetrics and Gynecology (산부인과학교실) 
Issue Date
Objective: The potential therapeutic effects of ginsenoside Rg3 on endometriosis and its target miRNAs were identified. Methods: An in vitro study using human endometrial stromal cells (HESCs) obtained from patients with endometriosis and an in vivo study using mouse endometriosis models were designed. HESCs were treated with Rg3-enhanced extract (Rg3E); real-time PCR, microarray profiling, transfection, and western blot were performed. Mouse endometriosis models were developed and supplemented with Rg3E for 8 weeks. Results: RNA levels of Ki-67, Col-1, CTGF, fibronectin, TGF-β1, MMP2 and 9 were significantly decreased in Rg3E-treated HESCs. Microarray analysis revealed downregulation of miR-27b-3p, which is related to fibrosis modulation. Expression of miR-27b-3p was significantly higher in HESCs from patients with endometriosis than that of controls, and Rg3E treatment significantly decreased its expression. The transfection of miR-27b inhibitor revealed significant decreases in Col-1 and MMP9. The protein expression results from western blot were also comparable. The contraction and migration assay revealed significant reductions in both fibrosis and migration potential in Rg3E-treated HESCs from endometriosis patients. In Rg3E-treated mice, decreases in size and fibrotic character of endometrial lesions were observed. Conclusion: Rg3E effectively alters fibrotic properties of HESCs from patients with endometriosis, which is presumed to be associated with miR-27b-3p modulation.
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1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 3. Dissertation
Yonsei Authors
Kim, Min Kyoung(김민경)
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