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Modification and evaluation of the Triton Hodge test for screening carbapenemase-producing Enterobacteriaceae

Authors
 Jung-Hyun Byun  ;  Jung Lim Gim  ;  Jong Hwa Yum  ;  Dongeun Yong  ;  Kyungwon Lee  ;  Yunsop Chong 
Citation
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.95(4) : 114872, 2019 
Journal Title
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 
ISSN
 0732-8893 
Issue Date
2019
Keywords
KPC ; Modified Hodge test ; NDM ; Phenotypic carbapenemase detection ; Triton Hodge test
Abstract
Detection of carbapenemase-producing Enterobacteriaceae (CPE) has become critical for appropriate antimicrobial therapy and for controlling the spread of infection. We evaluated Triton Hodge test (THT) for screening CPE. A spreader can be used to apply more constant volume of Triton on whole surface of Mueller-Hinton agar (MHA), or alternatively, a 10-μL inoculating loop can be used to apply a 20% Triton solution lineally. The THT procedure can be simplified by eliminating the 1/10 dilution step of indicator bacteria from the McFarland 0.5 turbidity suspension. The presence of Triton in the MHA plates significantly increased the enhanced growth size of not only Enterobacteriaceae producing NDM-1-like enzymes but also those producing the most prevalent KPC-2-like enzyme, resulting in 100% sensitivity of the test.
Full Text
https://www.sciencedirect.com/science/article/pii/S0732889318306199
DOI
10.1016/j.diagmicrobio.2019.114872
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Chong, Yun Sop(정윤섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174752
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