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Observation of Cell Division in a Fertilized Egg of a Zebrafish by Using a Multimodal Nonlinear Optical Microscope

Authors
 Sung-Ho Lee  ;  Seung-Hyun Hong  ;  Seung-Han Park  ;  Boram Kim  ;  Dong Hee Kim  ;  Seung Woo Park 
Citation
 JOURNAL OF THE KOREAN PHYSICAL SOCIETY, Vol.75(6) : 485-489, 2019 
Journal Title
 JOURNAL OF THE KOREAN PHYSICAL SOCIETY 
ISSN
 0374-4884 
Issue Date
2019
Keywords
Multimodal nonlinear optical microscopy ; Two-photon excitation microscopy ; Harmonic generation microscopy ; Zebrafish egg ; Somatic cell division
Abstract
We have developed a high-speed and high-resolution multimodal nonlinear optical microscope, which can acquire in vivo label and label-free 3D images. Using this microscope, we could successfully observe dynamic images of the zebrafish heartbeat with 33-millisecond intervals without staining process, indicating 1.68 beats per second. In addition, we show that the label-free, highspeed, real-time images can be obtained during somatic cell divisions in the zebrafish’s embryo.
Full Text
https://link.springer.com/article/10.3938%2Fjkps.75.485
DOI
10.3938/jkps.75.485
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Seung Woo(박승우) ORCID logo https://orcid.org/0000-0001-8230-964X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174712
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