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Mortalin is a distinct bio-marker and prognostic factor in serous ovarian carcinoma

Authors
 Ming Xu  ;  Tiefeng Jin  ;  Liyan Chen  ;  Xianglan Zhang  ;  Guang Zhu  ;  Qianrong Wang  ;  Zhenhua Lin 
Citation
 GENE, Vol.696 : 63-71, 2019 
Journal Title
 GENE 
ISSN
 0378-1119 
Issue Date
2019
MeSH
Adolescent ; Adult ; Aged ; Biomarkers, Tumor/metabolism* ; Carcinoma, Ovarian Epithelial/mortality ; Carcinoma, Ovarian Epithelial/pathology* ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation ; Computational Biology ; Datasets as Topic ; Epithelial-Mesenchymal Transition ; Female ; Follow-Up Studies ; HSP70 Heat-Shock Proteins/metabolism* ; Humans ; Kaplan-Meier Estimate ; Middle Aged ; Mitochondrial Proteins/metabolism* ; Ovarian Neoplasms/mortality ; Ovarian Neoplasms/pathology* ; Prognosis ; Retrospective Studies ; Survival Rate ; Wnt Signaling Pathway ; Young Adult
Keywords
Biomarker ; Mortalin ; Ovarian carcinoma ; Prognostic value ; Survival analysis
Abstract
This study focused on mortalin expression and its relevance to the prognosis in serous ovarian carcinoma, mortalin modulated cell malignant proliferation and EMT progression via Wnt/β-Catenin signaling pathway. In this study, data obtained from Oncomine database, Cancer Cell Line Encyclopedia (CCLE) analysis and Immunohistochemical (IHC) staining was used to assess the expression of mortalin in serous ovarian carcinoma. The prognostic value of mortalin was analyzed using Meier plotter database and Kaplan-Meier. MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay, immunofluorescence (IF) staining, and colony formation assay were used to detect cell reproductive capacity. SK-OV-3 cell motility and epithelial-mesenchymal transition (EMT) were measured by wound-healing, migration and western-blot assays. Data from Oncomine showed that mortalin was highly expressed in serous ovarian carcinomas compared with corresponding normal controls. Similar results were found in CCLE analysis and in clinical specimens. High mortalin expression was associated with high histological grade and worse overall survival (OS) rate. The results of MTT analyses, IF staining, and colony formation assay indicated that MKT-077 (1-Ethyl-2-[[3-ethyl-5-(3-methyl-2(3H)-benzothiazolylidene)-4-oxo-2-thiazolidinylidene] methyl]-pyridinium chloride) suppressed the viability of SK-OV-3 cells. Besides, mortalin suppression restrained cell EMT progression by Wnt/β-Catenin signaling pathway. Taken together, mortalin is over-expressed in serous ovarian carcinoma. High mortalin expression could be a candidate for the prognostic indicator and a biomarker in serous ovarian carcinoma.
Full Text
https://www.sciencedirect.com/science/article/pii/S0378111919301581
DOI
10.1016/j.gene.2019.02.033
Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
Yonsei Authors
Zhang, Xiang Lan(장향란)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174621
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