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Use of Droplet Digital Polymerase Chain Reaction for Detecting Minimal Residual Disease: A Prospective Multi-Institutional Study

Authors
 HYUNKYUNG PARK  ;  DONG-YEOP SHIN  ;  INHO KIM  ;  SANG-KYUN SOHN  ;  YOUNGIL KOH  ;  JE-HWAN LEE  ;  KYOO-HYUNG LEE  ;  DAE-YOUNG KIM  ;  HYEONG-JOON KIM  ;  JAE-SOOK AHN  ;  JEONG-OK LEE  ;  SOO-MEE BANG  ;  JUNE-WON CHEONG  ;  SANG-GON PARK  ;  SEONYANG PARK  ;  YOO JIN LEE  ;  SEO-YEON AHN 
Citation
 IN VIVO, Vol.33(6) : 2273-2280, 2019 
Journal Title
 IN VIVO 
ISSN
 0258-851X 
Issue Date
2019
Keywords
Chronic myeloid leukemia ; complete molecular response ; droplet digital polymerase chain reaction ; minimal residual disease ; treatment outcome
Abstract
BACKGROUND/AIM: Droplet digital polymerase chain reaction (ddPCR) is an exact method of measuring nucleic acids. The aim of this prospective study was to evaluate minimal residual disease (MRD) using ddPCR in chronic myeloid leukemia (CML) patients. PATIENTS AND METHODS: Between May 2013 and November 2014, CML patients treated with nilotinib were enrolled in our study. BCR/ABL1 transcripts levels were evaluated using ddPCR at the first time of complete molecular response (CMR). We enrolled 15 patients from 7 Institutions. The treatment period and median follow-up period were 45 months and 47 months, respectively. RESULTS: Patients with a high level of BCR/ABL1 transcript had a greater tendency to lose the CMR during the follow-up period (p=0.095). In addition, patients with a low level of BCR/ABL1 transcript showed a longer duration of CMR compared to those with a high level (p=0.032). CONCLUSION: We found that ddPCR is a sensitive method for detecting MRD and that MRD could affect the duration of the treatment response.
Files in This Item:
T201905040.pdf Download
DOI
10.21873/invivo.11733
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Cheong, June-Won(정준원) ORCID logo https://orcid.org/0000-0002-1744-0921
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174617
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