Cited 23 times in
Sequential cleavage of poly(ADP-ribose)polymerase and appearance of a small Bax-immunoreactive protein are blocked by Bcl-X(L) and caspase inhibitors during staurosporine-induced dopaminergic neuronal apoptosis
DC Field | Value | Language |
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dc.contributor.author | 장인익 | - |
dc.date.accessioned | 2020-01-23T05:37:33Z | - |
dc.date.available | 2020-01-23T05:37:33Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 0022-3042 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/174415 | - |
dc.description.abstract | To assess the role of Bcl-X(L) and its splice derivative, Bcl-X(S), in staurosporine-induced cell death, we used a dopaminergic cell line, MN9D, transfected with bcl-xL (MN9D/Bcl-X(L)), bcl-xS (MN9D/Bcl-X(S)), or control vector (MN9D/Neo). Only 8.6% of MN9D/Neo cells survived after 24 h of 1 microM staurosporine treatment. Caspase activity was implicated because a caspase inhibitor, N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-fmk), attenuated staurosporine-induced cell death. Bcl-X(L) rescued MN9D cells from death (89.4% viable cells), whereas Bcl-X(S) had little or no effect. Bcl-X(L) prevented morphologically apoptotic changes as well as cleavage of poly(ADP-ribose)polymerase (PARP) induced by staurosporine. It is interesting that a small Bax-immunoreactive protein appeared 4-8 h after PARP cleavage in MN9D/Neo cells. The appearance of the small Bax-immunoreactive protein, however, may be cell type-specific as it was not observed in PC12 cells after staurosporine treatment. The sequential cleavage of PARP and the appearance of the small Bax-immunoreactive protein in MN9D cells were blocked either by Z-VAD-fmk or by Bcl-X(L). Thus, our present study suggests that Bcl-X(L) but not Bcl-X(S) prevents staurosporine-induced apoptosis by inhibiting the caspase activation that may be directly or indirectly responsible for the appearance of the small Bax-immunoreactive protein in some types of neurons. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Wiley | - |
dc.relation.isPartOf | Journal of Neurochemistry | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis/drug effects | - |
dc.subject.MESH | Apoptosis/physiology* | - |
dc.subject.MESH | Caspase Inhibitors* | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cycloheximide/pharmacology | - |
dc.subject.MESH | Dopamine/metabolism* | - |
dc.subject.MESH | Kinetics | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Neurons/cytology | - |
dc.subject.MESH | Neurons/drug effects | - |
dc.subject.MESH | Neurons/metabolism* | - |
dc.subject.MESH | Poly(ADP-ribose) Polymerases/metabolism* | - |
dc.subject.MESH | Proto-Oncogene Proteins/metabolism* | - |
dc.subject.MESH | Proto-Oncogene Proteins c-bcl-2/biosynthesis | - |
dc.subject.MESH | Proto-Oncogene Proteins c-bcl-2/genetics | - |
dc.subject.MESH | Proto-Oncogene Proteins c-bcl-2/metabolism* | - |
dc.subject.MESH | Recombinant Proteins/biosynthesis | - |
dc.subject.MESH | Recombinant Proteins/metabolism | - |
dc.subject.MESH | Staurosporine/pharmacology* | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | bcl-2-Associated X Protein | - |
dc.subject.MESH | bcl-X Protein | - |
dc.title | Sequential cleavage of poly(ADP-ribose)polymerase and appearance of a small Bax-immunoreactive protein are blocked by Bcl-X(L) and caspase inhibitors during staurosporine-induced dopaminergic neuronal apoptosis | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학교실) | - |
dc.contributor.googleauthor | Ji-Eun Kim | - |
dc.contributor.googleauthor | Jae H. Oh | - |
dc.contributor.googleauthor | Won-Seok Choi | - |
dc.contributor.googleauthor | In I. Chang | - |
dc.contributor.googleauthor | Seonghyang Sohn | - |
dc.contributor.googleauthor | Stanislaw Krajewski | - |
dc.contributor.googleauthor | John C. Reed | - |
dc.contributor.googleauthor | Karen L. O’Malley | - |
dc.contributor.googleauthor | Young J. Oh | - |
dc.identifier.doi | 10.1046/j.1471-4159.1999.0722456.x | - |
dc.contributor.localId | A03461 | - |
dc.relation.journalcode | J01620 | - |
dc.identifier.eissn | 1471-4159 | - |
dc.identifier.pmid | 10349855 | - |
dc.contributor.alternativeName | Chang, In Ik | - |
dc.contributor.affiliatedAuthor | 장인익 | - |
dc.citation.volume | 72 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 2456 | - |
dc.citation.endPage | 2463 | - |
dc.identifier.bibliographicCitation | Journal of Neurochemistry, Vol.72(6) : 2456-2463, 1999 | - |
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