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Monoclonal antibodies to recombinant Der p 2, a major house dust mite allergen: specificity, epitope analysis and development of two-site capture ELISA

 Tai-Soon Yong  ;  Sang-Mi Lee  ;  Gab-Man Park  ;  In-Yong Lee  ;  Han-Il Ree  ;  Kyung-Sup Kim  ;  Sang-Hwan Oh  ;  Jung-Won Park  ;  Chein-Soo Hong 
 Korean Journal of Parasitology, Vol.37(3) : 163-169, 1999 
Journal Title
 Korean Journal of Parasitology 
Issue Date
Animals ; Antibodies, Monoclonal* ; Antigens, Dermatophagoides ; Enzyme-Linked Immunosorbent Assay/methods* ; Epitopes/analysis* ; Glycoproteins/analysis* ; Glycoproteins/immunology* ; Glycoproteins/standards ; Mites/immunology* ; Recombinant Proteins ; Sensitivity and Specificity ; Species Specificity
House dust mite allergens have been well established as sensitizing agents that are important in the induction of allergic diseases. In order to analyze epitopes of the allergen and to develop a quantitative method of the allergen exposure, monoclonal antibodies against a recombinant Der p 2 (rDer p 2), one of the major allergens of Dermatophagoides pteronyssinus, were produced. Four monoclonal antibodies produced were species-specific and did not cross-react to the D. farinae crude extract. Two of the monoclonal antibodies were found to be IgG1 and the others were IgM. For the analysis of epitopes, a Der p 2 cDNA encoding 126 amino acids (aa) was dissected into three fragments with several overlapping peptides. A (aa residues 1-49), B (44-93), and C fragment (84-126). Three monoclonal antibodies showed reactivities to the recombinant B fragment and to the full-length rDer p 2, but one monoclonal antibody reacted only with the full-length rDer p 2. Two-site capture ELISA was developed using two different monoclonal antibodies for quantitating Der p 2 in house dust. The sensitivity limit was 4 ng/ml with rDer p 2 and 8 micrograms/ml with the D. pteronyssinus crude extract. The result suggested that the assay using monoclonal antibodies against rDer p 2 could be useful for the environmental studies and for the standardization of mite allergen extracts.
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1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
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