22 53

Cited 0 times in

Synthesized TGF-βs in RPE regulates cellular proliferation

DC FieldValueLanguage
dc.contributor.author성공제-
dc.date.accessioned2020-01-23T05:25:39Z-
dc.date.available2020-01-23T05:25:39Z-
dc.date.issued1999-
dc.identifier.issn1011-8942-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/174269-
dc.description.abstractRetinal pigment epithelial (RPE) cells transdifferentiate in culture, a transition which is accompanied by a shift in biological activity. The present study investigates whether transforming growth factor (TGF)-β has the same effects on morphologically transformed RPE cells that it has on primary RPE cells. It also evaluates the autocrine and paracrine activities of TGF-βs synthesized by RPE cells as well as the anti-TGF-β effect of mannose-6-phosphate (M-6-P). RPE cells were subcultured at the sixth passage to induce morphological change. The effect of second passaged RPE-conditioned medium (CM) on DNA synthesis was evaluated by the incorporation of ³H-thymidine in rabbit subconjunctival fibroblasts (SCFs) and primary RPE cells. The presence of TGF-β in RPE-CM was determined using immunoblotting analysis. And the inhibitory effect of M-6-P on cell proliferation mediated by RPE-CM was also analyzed using ³H-thymidine incorporation into DNA. TGF-β1, TGF-β2, and TGF-β3 inhibited the proliferation of the primary cultures of RPE cells in a dose-dependent manner, but the spindle-shaped sixth passaged RPE cells were not inhibited by these growth factors. The medium conditioned by RPE cells stimulated the proliferation of SCFs and inhibited the proliferation of primary RPE cells, in a manner similar to TGF-β. When this medium was precipitated with either anti-TGF-β1, anti-TGF-β2, or anti-TGF-β3 antibodies, all three TGF-βs, with an apparent molecular size of 25 kDa, were detected. Mannose-6-phosphate significantly blocked the effect of RPE-CM on cell proliferation. These findings indicate that RPE cells produce biologically functional TGF-βs and that M-6-P can block the inhibitory effect of RPE-CM on cell proliferation.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherKJO-
dc.relation.isPartOfKorean Journal of Ophthalmology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleSynthesized TGF-βs in RPE regulates cellular proliferation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Ophthalmology (안과학교실)-
dc.contributor.googleauthorSung Chul Lee-
dc.contributor.googleauthorGong Je Seong-
dc.contributor.googleauthorSoon Hyun Kim-
dc.contributor.googleauthorOh Woong Kwon-
dc.contributor.localIdA01946-
dc.relation.journalcodeJ02931-
dc.identifier.eissn2092-9382-
dc.contributor.alternativeNameSeong, Gong Je-
dc.contributor.affiliatedAuthor성공제-
dc.citation.volume13-
dc.citation.number1-
dc.citation.startPage16-
dc.citation.endPage24-
dc.identifier.bibliographicCitationKorean Journal of Ophthalmology, Vol.13(1) : 16-24, 1999-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.