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메밀의 알레르기 항원성에 관한 연구

Other Titles
 Identification and characterization of buckwheat allergen 
Authors
 정병주  ;  류정우  ;  염혜영  ;  김규언  ;  박중원  ;  홍천수  ;  이기영 
Citation
 Journal of Asthma, Allergy and Clinical Immunology (천식 및 알레르기), Vol.19(1) : 62-69, 1999 
Journal Title
 Journal of Asthma, Allergy and Clinical Immunology (천식 및 알레르기) 
ISSN
 1226-8739 
Issue Date
1999
Keywords
buckwheat ; major allergen ; glycosylation
Abstract
Background and Objective: Buckwheat is considered one of the most important food allergens in Korea. Although a very small amount is ingested or inhaled, it can cause seri- ous allergic reactions. However, the major allergens of buckwheat still remain to be eluci- dated. The aim of our study was to identify and characterize the major allergen of buck- wheat seed. Material and method: Dialysis membrane with a cut-off MW 1kD was used for the preparation of crude buckwheat seed allergen extract. SDS-PAGE under reducing condi- tions and IgE immunoblotting were performed using sera from 15 buckwheat sensitive sub- jects. Isoelectric focusing and lectin blotting assay were done. Result: Western blot analysis showed more than 15 IgE-reactive buckwheat proteins. Among them, a 24kD protein was shown to be the most frequently bound to sera from allergic subjects (54%). Isoelectric point of 24kD protein was around 5.9. In lectin blotting assay, 24kD protein did not bind to Con A nor five other lectins. Conclusion: A 24kD protein was the most frequently recognized allergenic component in buckwheat seed. Isoelectric point was around 5.9. Glycosylation was not detected in 24kD of buckwheat protein.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Hong, Chein Soo(홍천수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174243
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