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Methicillin 내성 포도구균의 mecI, mecA 및 femA 유전자에 관한 연구

Other Titles
 A Study of the mecI, mecA and femA Genes of Methicillin-Resistant Staphylococci 
Authors
 안지영  ;  김원배  ;  이동화  ;  이경원  ;  최석호  ;  김일수  ;  서채형 
Citation
 Korean Journal of Clinical Pathology (대한임상병리학회지), Vol.19(1) : 62-69, 1999 
Journal Title
 Korean Journal of Clinical Pathology (대한임상병리학회지) 
ISSN
 1015-6445 
Issue Date
1999
Keywords
Staphylococcus aureus ; Staphylococcus epidermidis ; Agar dilution ; Polymerase chain reaction ; Comparative study ; mecI gene ; mecA gene ; femA gene
Abstract
Background :Staphylococcus aureus and Staphylococcus epidermidis are those of the important pathogens that have revealed the increase of methicillin resistance. Because of increasing prevalence of staphylococci resistant to one or more antimicrobial agents, it is necessary to determine the antibiotic susceptibility of these microorganisms. Methicillin resistance is due to the production of PBP2′, which is encoded by mecA gene in the chromosome. PBP 2′shows low affinity to the all of -lactam drugs. Therefore, the determination of gene is considered as a correct method for the antibiotic treatment procedures. Method :In order to examine effectiveness of detecting mecA and femA genes for the identification of methicillin-resistant S. aureus (MRSA) and mecI gene in high-level-resistant MRSA, the presence of these genes in S. aureus and S. epidermidis was investigated by PCR. The types of genes were compared with phenotype by agar dilution method and investigated with MIC of methicillin. Result :1. The mecA gene detection was useful for the identification of MRSA with MRSA 100% and methicillin-susceptable S. aureus (MSSA) 2.7% (P0.001). 2. The femA gene detection was useful for the S. aureus identification (P0.001). Conclusion :The study concluded that the detection of both the mecA and femA genes from staphylococci by the PCR method had been considered as a correct and useful method in the identification of MRSA. mecI gene has been deemed as a repressor gene for high-level-resistant MRSA that is clinically useful as a standard. However, it is considered that the investigation should be done with later detected nucleotide sequencing of the mecI gene.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174219
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