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Pathogenicity of Korean isolates of Acanthamoeba by observing the experimental infection and zymodemes of five isoenzymes

Authors
 Kyung-Il Im  ;  Ho-Joon Shin  ;  Dong Whan Seo  ;  Soung-Hoo Jeon  ;  Tae-Eun Kim 
Citation
 Korean Journal of Parasitology, Vol.37(2) : 85-92, 1999 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
1999
MeSH
Acanthamoeba/enzymology ; Acanthamoeba/pathogenicity* ; Amebiasis/parasitology* ; Animals ; Aspartate Aminotransferases/isolation & purification ; CHO Cells ; Cricetinae ; Glucosephosphate Dehydrogenase/isolation & purification ; Hexokinase/isolation & purification ; Isoenzymes/isolation & purification* ; Korea ; Malate Dehydrogenase/isolation & purification ; Mice ; Mice, Inbred ICR ; Virulence
Abstract
To determine the pathogenicity of Acanthamoeba spp. isolated in Korea and to develop a isoenzymatic maker, the mortality rate of infected mice, in vitro cytotoxicity against target cells and isoenzyme band patterns were observed. Five isolates of Acanthamoeba spp. (YM-2, YM-3, YM-4, YM-5, and YM-7) were used in this study as well as three reference Acanthamoeba spp. (A. culbertsoni, A. hatchetti, and A. royreba). According to the mortality rate of infected mice, Korean isolates could be categorized into three groups high virulent (YM-4), low virulent (YM-2, YM-5, YM-7) and the nonpathogenic group (YM-3). In addition, the virulence of Acanthamoeba spp. was enhanced by brain passage in mice. In the cytotoxicity assay against chinese hamster ovary cells, especially, the cytotoxicity of brain-passaged amoebae was relatively higher than the long-term cultivated ones. The zymodeme patterns of glucose-6-phosphate dehydrogenase (G6PD), malate dehydrogenase (MDH), hexokinase (HK), glutamate oxaloacetate transaminase (GOT) and malic enzyme (ME) of Acanthamoeba spp. were different among each isolate, and also between long-term cultured amoebae and brain passaged ones. In spite of the polymorphic zymodemes, a slow band of G6PD and HK, and an intermediate band of MDH were only observed in pathogenic Acanthamoeba spp., which should be used as isoenzymatic makers.
Files in This Item:
T199902041.pdf Download
DOI
10.3347/kjp.1999.37.2.85
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Im, Kyung Il(임경일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174114
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