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국내 일부지역 설사환자의 로타바이러스 감염실태 및 분리동정

Other Titles
 Prevalence and Identification of Rotaviruses in Stool Specimens of Patients with Acute Diarrhea from Several Regions of Korea 
 김동수  ;  박범수  ;  정동혁  ;  안재문  ;  김철중  ;  강신영 
 Journal of the Korean Pediatric Association (소아과), Vol.42(4) : 501-509, 1999 
Journal Title
Journal of the Korean Pediatric Association(소아과)
Issue Date
Rota virus ; Electropherotype ; Serotype
Purpose : Rotaviruses are the main cause of infantile diarrheal diseases worldwide. The purpose of this study is to obtain epidemiologic data of rotavirus infections in Korea.
Methods : Stool specimens were collected from 150 patients with acute diarrheal symptoms, who were admitted to Yonsei Medical Center and Chungbuk National University Hospital. After isolating the virus from the specimens, the viruses were identified as rotaviruses by electron microscope and fluorescent microscope after staining with rotavirus VP6-specific monoclonal antibody. RNA was extracted from the specimens by modified phenol/chloroform method. Electropherotying was done with extracted RNA samples after silver staining of the gels. Rotavirus serotyping was done using commercial serotyping ELISA kit.
Results : Rate of infection from rotavirus stool samples was 60%. Isolated rotaviruses were all serogroup A and a majority(46.0%) of these viruses were long type. Serotypes 1 and 2 were identified with serotype 1 being the majority(52.2%). There were no differences in these types between 1991 and 1992, and also between Seoul and Cheongju area.
Conclusion : Electropherotype of rotavirus epidemic in Korea was serogroup A, and long type in the majority. Serotypes of rotavirus epidemic in Korea were type 1 and type 2. These results show that the rotavirus reassortant vaccine developed in America could also handle the rotaviral disease in Korea.
Files in This Item:
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1. College of Medicine (의과대학) > Dept. of Pediatrics (소아과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Soo(김동수)
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