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거세된 토끼에서 남성호르몬투여가 음경의 Nitric Oxide Synthase에 미치는 영향

Other Titles
 The Effect of Testosterone on Nitric Oxide Synthase in the Penis of the Rabbit 
 최영득  ;  정우식 
 Korean Journal of Urology (대한비뇨기과학회지), Vol.40(1) : 106-112, 1999 
Journal Title
Issue Date
NITRIC OXIDE SYNTHASE ; Testosterone ; Nitric oxide ; Cavernosal smooth muscle ; Rabbit
Purpose: Androgen was thought to be linked to sexual activity in man but its
peripheral action have not been identified precisely. Recently, androgens dependent nature
of nitric oxide synthase(NOS), the enzyme system producing nitric oxide(NO) as the
principal mediator in penile erection, has been studied. The present study was designed
to determine if NO within the penis is regulated by androgen.
Materials and Methods: Effect of testosterone on the penile NO was investigated from
three groups of rabbit; control(a sham operation), castration group(four sub-groups: 1, 2,
3 and 4 weeks after castration) and testosterone replacement group(four sub-groups:
testosterone propionate(10㎎/day) was given subcutaneously from 1, 2, 3 and 4 weeks
after castration for 7 days respectively). Strips of rabbit corpus cavernosum were
isolated and mounted in organ chambers. The relaxation effects were assessed with
electrical field stimulation(EFS) in the precontracted cavernosal muscle strips with
phenylephrine. Penile NOS activity was measured by the rate of
14C-L-arginine-citrulline conversion. Penile tissue was subsequently
stained for the presence of NOS, using a nicotinamide adenine dinucleotide phosphate
diaphorase(NADPH-d) histochemistry.
Results: EFS evoked a relaxation of rabbit corpus cavernosum. A gradual decrease in
the EFS evoked relaxation was recorded, proportional to the duration of being castrated,
which was restored to the level of control by testosterone replacement. After castration,
penile NOS activity was significantly reduced to 51.3%, 43.7%, 37.5%, and 32.7% of the
level of control at 1, 2, 3, 4 weeks. This decrease in penile NOS activity after castration
was also restored by testosterone replacement. Diffusely scattered delicate nerve fibers
showing blue color reaction after NADPH-d histochemical staining were reduced in
castrated cavernosum in comparison with control.
Conclusions: Based on presented data we conclude that testosterone plays a direct role
in penile erection by regulating the activity of NOS within the penis.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers
Yonsei Authors
Choi, Young Deuk(최영득) ORCID logo https://orcid.org/0000-0002-8545-5797
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