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Helicobater pylori에 의한 위암세포주 사멸

DC FieldValueLanguage
dc.contributor.author김원호-
dc.contributor.author여말희-
dc.contributor.author이경원-
dc.contributor.author이용찬-
dc.date.accessioned2020-01-10T02:08:29Z-
dc.date.available2020-01-10T02:08:29Z-
dc.date.issued1999-
dc.identifier.issn1598-9992-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/173902-
dc.description.abstractBackground/Aims: Helicobacter pylori (H. pylori) is associated with active gastritis and peptic ulcer disease. Mechanism for H. pylori-induced gastric epithelial damage is still incompletely understood. However, the increase of apoptotic cells in H. pylori-infected mucosa suggested that apoptosis could be a major mechanism for cellular damage. As an effort to clarify the mechanism, we investigated whether H. pylori directly induce apoptosis in gastric cancer cells in vitro. Methods: Cultured H. pylori (ATCC 43504) were suspended as 109/mL. IL (interleukin)-8 was measured by enzyme linked immunosorbent assay. Cell survival was assessed by MTT [3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. Apoptosis was detected and confirmed by demonstration of DNA fragmentation and morphologic changes. Results: H. pylori induced IL-8 production as well as decrease of cell survival in gastric cancer cell lines in a time- and concentration-dependen way. Addition of H. pylori to gastric cancer cells induced apoptosis. Such induction was not organ specific. Heat or formalin treatment of H. pylori almost completely inhibited IL-8 production but only partially blocked apoptosis. H. pylori- induced apoptosis was potentiated by interferon-γ pretreatment in HT-29 but not in AGS and KATO III. Conclusions: These results suggest that H. pylori affects on gastric epithelial cell growth by direct induction of apoptosis.-
dc.description.statementOfResponsibilityopen-
dc.languageKorean-
dc.publisher대한소화기학회-
dc.relation.isPartOfKorean Journal of Gastroenterology (대한소화기학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleHelicobater pylori에 의한 위암세포주 사멸-
dc.title.alternativeHelicobacter pylori-induced Apoptosis in Gastric Cancer Cell Lines-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthor김원호-
dc.contributor.googleauthor여말희-
dc.contributor.googleauthor이꽃실-
dc.contributor.googleauthor이용찬-
dc.contributor.googleauthor박인서-
dc.contributor.googleauthor이경원-
dc.contributor.localIdA00774-
dc.contributor.localIdA02345-
dc.contributor.localIdA02345-
dc.contributor.localIdA02649-
dc.contributor.localIdA02649-
dc.contributor.localIdA02988-
dc.contributor.localIdA02988-
dc.relation.journalcodeJ02015-
dc.identifier.eissn2233-6869-
dc.subject.keywordHelicobacter pylori-
dc.subject.keywordApoptosis-
dc.subject.keywordInterleukin-8-
dc.contributor.alternativeNameKim, Won Ho-
dc.contributor.affiliatedAuthor김원호-
dc.contributor.affiliatedAuthor여말희-
dc.contributor.affiliatedAuthor여말희-
dc.contributor.affiliatedAuthor이경원-
dc.contributor.affiliatedAuthor이경원-
dc.contributor.affiliatedAuthor이용찬-
dc.contributor.affiliatedAuthor이용찬-
dc.citation.volume34-
dc.citation.number1-
dc.citation.startPage21-
dc.citation.endPage34-
dc.identifier.bibliographicCitationKorean Journal of Gastroenterology (대한소화기학회지), Vol.34(1) : 21-34, 1999-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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