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Use of Liquid Chromatography-Tandem Mass Spectrometry for Clinical Testing in Korean Laboratories: a Questionnaire Survey

Authors
 Hyojin Chae  ;  Sung-Eun Cho  ;  Hyung-Doo Park  ;  Sail Chun  ;  Yong-Wha Lee  ;  Yeo-Min Yun  ;  Sang-Hoon Song  ;  Sang-Guk Lee  ;  Kyunghoon Lee  ;  Junghan Song  ;  Soo-Youn Lee 
Citation
 ANNALS OF LABORATORY MEDICINE, Vol.39(5) : 447-453, 2019 
Journal Title
ANNALS OF LABORATORY MEDICINE
ISSN
 2234-3806 
Issue Date
2019
MeSH
Chromatography, High Pressure Liquid/standards* ; Laboratories, Hospital/standards* ; Metanephrine/blood ; Quality Control ; Republic of Korea ; Surveys and Questionnaires ; Tandem Mass Spectrometry/standards* ; Vitamin D/analysis
Keywords
Harmonization ; Korea ; Liquid chromatography-tandem mass spectrometry ; Survey
Abstract
BACKGROUND: The use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) has substantially increased in clinical laboratories worldwide. To assess the status of clinical LC-MS/MS testing in Korean laboratories, a questionnaire survey was performed by the Clinical Mass Spectrometry Research Committee of the Korean Society of Clinical Chemistry.

METHODS: The questionnaire was distributed to 19 clinical laboratories performing clinical LC-MS/MS from April to May 2018. It asked about general characteristics of the laboratory and commonly utilized clinical LC-MS/MS tests: newborn screening, tacrolimus test, vitamin D test, and plasma metanephrine test. Frequency analysis and other statistical analyses were performed.

RESULTS: A total of 17 laboratories responded. The median number of LC-MS/MS instruments, laboratory medicine physicians, and technicians in each laboratory was three, one, and two, respectively. Nine laboratory directors had >10 years of experience with clinical LC-MS/MS. For each LC-MS/MS test, at least two concentrations of QC materials were measured every 24 hours during clinical testing, and all laboratories used QC acceptability criteria based on their established QC means and SDs. All laboratories participated in an external quality assessment program. However, there was inter-laboratory variability in sample preparation methods, instruments, reagents, internal standards, and calibrators.

CONCLUSIONS: LC-MS/MS has been successfully introduced in Korean clinical laboratories and is used within a quality framework. Further efforts for harmonization on a nationwide basis could facilitate the widespread use of LC-MS/MS.
Files in This Item:
T201904564.pdf Download
DOI
10.3343/alm.2019.39.5.447
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Sang-Guk(이상국) ORCID logo https://orcid.org/0000-0003-3862-3660
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/173386
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