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Performance evaluation of the PANA RealTyper™ CRE Kit for detecting carbapenemase genes in Gram-negative bacilli

Authors
 Jun Sung Hong  ;  Dokyun Kim  ;  Eun-Jeong Yoon  ;  Hyukmin Lee  ;  Seok Hoon Jeong 
Citation
 JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, Vol.18 : 100-103, 2019 
Journal Title
 JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE 
ISSN
 2213-7165 
Issue Date
2019
Keywords
Carbapenemase ; Carbapenemase-producing organism ; Evaluation ; Gram-negative bacilli ; Real-time PCR
Abstract
OBJECTIVES: The spread of carbapenemase-producing organisms has been continuously reported over the past decade. Rapid and accurate detection of carbapenemase production is essential for adequate infection control and appropriate antimicrobial treatment in the clinical setting. In this study, the performance of the newly developed PANA RealTyper™ CRE Kit (PANAGENE, Daejeon, South Korea) for the detection of six common carbapenemase genes (blaKPC, blaGES, blaIMP, blaNDM, blaVIM and blaOXA-48-like) was evaluated. METHODS: A total of 479 non-duplicate clinical isolates of Gram-negative bacilli, including 391 carbapenemase-producers and 88 non-producers, were tested. Conventional PCR and sequencing were performed as reference methods for performance evaluation of the PANA RealTyper™ CRE Kit. RESULTS: The PANA RealTyper™ CRE Kit showed a reliable performance for the detection of carbapenemase-producing organisms compared with the monoplex PCR system, with sensitivity and specificity values both >95%, with only a few discrepant results for six types of carbapenemase genes. CONCLUSION: This kit is rapid and accurate for simultaneously detecting various carbapenemase genes in a single reaction and could contribute to early decisions for appropriate antimicrobial treatment in the clinical setting.
Full Text
https://www.sciencedirect.com/science/article/pii/S2213716519300426
DOI
10.1016/j.jgar.2019.02.002
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dokyun(김도균) ORCID logo https://orcid.org/0000-0002-0348-5440
Yoon, Eun-Jeong(윤은정)
Lee, Hyuk Min(이혁민) ORCID logo https://orcid.org/0000-0002-8523-4126
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
Hong, Jun Sung(홍준성)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/173293
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