Cited 17 times in
PPE39 of the Mycobacterium tuberculosis strain Beijing/K induces Th1-cell polarization through dendritic cell maturation
DC Field | Value | Language |
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dc.contributor.author | 신성재 | - |
dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2019-12-18T00:27:09Z | - |
dc.date.available | 2019-12-18T00:27:09Z | - |
dc.date.issued | 2019 | - |
dc.identifier.issn | 0021-9533 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/173052 | - |
dc.description.abstract | In a previous study, we have identified MTBK_24820, the complete protein form of PPE39 in the hypervirulent Mycobacterium tuberculosis (Mtb) strain Beijing/K by using comparative genomic analysis. PPE39 exhibited vaccine potential against Mtb challenge in a murine model. Thus, in this present study, we characterize PPE39-induced immunological features by investigating the interaction of PPE39 with dendritic cells (DCs). PPE39-treated DCs display reduced dextran uptake and enhanced MHC-I, MHC-II, CD80 and CD86 expression, indicating that this PPE protein induces phenotypic DC maturation. In addition, PPE39-treated DCs produce TNF-α, IL-6 and IL-12p70 to a similar and/or greater extent than lipopolysaccharide-treated DCs in a dose-dependent manner. The activating effect of PPE39 on DCs was mediated by TLR4 through downstream MAPK and NF-κB signaling pathways. Moreover, PPE39-treated DCs promoted naïve CD4+ T-cell proliferation accompanied by remarkable increases of IFN-γ and IL-2 secretion levels, and an increase in the Th1-related transcription factor T-bet but not in Th2-associated expression of GATA-3, suggesting that PPE39 induces Th1-type T-cell responses through DC activation. Collectively, the results indicate that the complete form of PPE39 is a so-far-unknown TLR4 agonist that induces Th1-cell biased immune responses by interacting with DCs.This article has an associated First Person interview with the first author of the paper. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Company of Biologists | - |
dc.relation.isPartOf | JOURNAL OF CELL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | PPE39 of the Mycobacterium tuberculosis strain Beijing/K induces Th1-cell polarization through dendritic cell maturation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | Hong-Hee Choi | - |
dc.contributor.googleauthor | Kee Woong Kwon | - |
dc.contributor.googleauthor | Seung Jung Han | - |
dc.contributor.googleauthor | Soon Myung Kang | - |
dc.contributor.googleauthor | Eunsol Choi | - |
dc.contributor.googleauthor | Ahreum Kim | - |
dc.contributor.googleauthor | Sang-Nae Cho | - |
dc.contributor.googleauthor | Sung Jae Shin | - |
dc.identifier.doi | 10.1242/jcs.228700 | - |
dc.contributor.localId | A02114 | - |
dc.contributor.localId | A03824 | - |
dc.relation.journalcode | J01301 | - |
dc.identifier.eissn | 1477-9137 | - |
dc.identifier.pmid | 31371491 | - |
dc.identifier.url | https://jcs.biologists.org/content/132/17/jcs228700.long | - |
dc.subject.keyword | Beijing/K strain | - |
dc.subject.keyword | Dendritic cell | - |
dc.subject.keyword | Mycobacterium tuberculosis | - |
dc.subject.keyword | PPE39 | - |
dc.subject.keyword | TLR4 | - |
dc.subject.keyword | Th1-polarization | - |
dc.contributor.alternativeName | Shin, Sung Jae | - |
dc.contributor.affiliatedAuthor | 신성재 | - |
dc.contributor.affiliatedAuthor | 조상래 | - |
dc.citation.volume | 132 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 14 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELL SCIENCE, Vol.132(17) : 1-14, 2019 | - |
dc.identifier.rimsid | 63788 | - |
dc.type.rims | ART | - |
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