알레르기 질환을 앓고 있는 소아에서 Wetern-Blot을 이용한 D.pteronyssinus 조항원에대한 IgE항체결합 양상 비교

Abstract

House dust mite is the most important causative allergens of childhood asthma and its positive rate of allergy skin test reaches up to 80% in atopic asthmatic children in Korea. Although the mite sensitization is more significant in older children(>4 years), it is also important in some younger(<4 years) asthmatic children. To identify the major allergens in Korean atopic asthmatic children and find the variations of IgE binding pattern to Dermatoph agoides pteronyssinus(Dp) in younger and older age groups, we conducted IgE immunoblot. Sera were collected in 25 children with Dp-sensitive atopic asthma : Group Ⅰ(younger children aged 1-3), Group Ⅱ(older children aged 4-12). Using our own-made crude Dp extract, we conducted IgE- immunoblot (biotin-avidine-phosphatase system). In the group Ⅰ, the 15KD protein was significantly bound by 69.2%(9/13) of sera and 98KD, 59KD, 31KD, 25KD components were bound by 8-16% of sera, respectively. In the group Ⅱ, the 15KD and 25KD proteins were significantly bound Immunoblot by 100% and 67% of sera, and 18KD, 94KD by 33%, 98KD by 25%, 45KD by 8% of sera, respectively. In conclusion, the 15KD protein is the most prevalent IgE-binding antigen in all age groups, on the other hands, IgE-sensitization to 25KD component is confirmed mainly in the cases of older age group in this study.