Cited 5 times in
Reconstitution of killer cell inhibitory receptor-mediated signal transduction machinery in a cell-free model system
DC Field | Value | Language |
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dc.contributor.author | 김종선 | - |
dc.contributor.author | 박채규 | - |
dc.date.accessioned | 2019-11-26T01:18:25Z | - |
dc.date.available | 2019-11-26T01:18:25Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 0003-9861 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/172915 | - |
dc.description.abstract | Recognition of class I MHC molecules on target cells by killer cell inhibitory receptors (KIRs) blocks natural cytotoxicity and antibody-dependent cell cytotoxicity of NK cells and CD3/TCR dependent cytotoxicity of T cells. The inhibitory effect of KIR ligation requires phosphorylation of the cytoplasmic tail of KIR and subsequent recruitment of an SH2-containing protein tyrosine phosphatase, SHP-1. To better understand the molecular mechanism of the KIR-mediated inhibitory signal transduction, we developed an in vitro assay system using a purified His-tag fusion protein of KIR cytoplasmic tail (His-CytKIR) and Jurkat T cell lysates. We identified a target molecule of SHP-1 by comparing the phosphorylation of major cellular substrates following in vitro phosphorylation of Jurkat cell lysates in the presence and absence of the His-CytKIR in this cell-free model system. The His-CytKIR was tyrosine phosphorylated by Lck in vitro, and the phosphorylated His-CytKIR recruited SHP-1. Interestingly, we observed that among major substrates phosphorylated in vitro, PLC-gamma exhibited a dramatic decrease in phosphorylation when the His-CytKIR was mixed with Jurkat T cell lysates. However, PLC-gamma exhibited no decrease in phosphorylation when SHP-1 or Lck was depleted or deficient in this reaction mixture, suggesting that the SHP-1 recruited by the phosphorylated His-CytKIR directly mediate the dephosphorylation of PLC-gamma. The cell-free model system could be used to reveal the detailed molecular interactions in the KIR-mediated signal transduction. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Academic Press | - |
dc.relation.isPartOf | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Biological Assay | - |
dc.subject.MESH | Cell-Free System | - |
dc.subject.MESH | Cytotoxicity, Immunologic | - |
dc.subject.MESH | Histocompatibility Antigens Class I/immunology | - |
dc.subject.MESH | Histocompatibility Antigens Class I/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intracellular Signaling Peptides and Proteins | - |
dc.subject.MESH | Jurkat Cells | - |
dc.subject.MESH | Killer Cells, Natural/immunology | - |
dc.subject.MESH | Killer Cells, Natural/metabolism* | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Protein Tyrosine Phosphatase, Non-Receptor Type 11 | - |
dc.subject.MESH | Protein Tyrosine Phosphatase, Non-Receptor Type 6 | - |
dc.subject.MESH | Protein Tyrosine Phosphatases/immunology | - |
dc.subject.MESH | Protein Tyrosine Phosphatases/metabolism* | - |
dc.subject.MESH | Receptors, Immunologic/genetics | - |
dc.subject.MESH | Receptors, Immunologic/immunology | - |
dc.subject.MESH | Receptors, Immunologic/metabolism* | - |
dc.subject.MESH | Receptors, KIR | - |
dc.subject.MESH | Recombinant Fusion Proteins/genetics | - |
dc.subject.MESH | Recombinant Fusion Proteins/immunology | - |
dc.subject.MESH | Recombinant Fusion Proteins/metabolism | - |
dc.subject.MESH | Signal Transduction/immunology* | - |
dc.title | Reconstitution of killer cell inhibitory receptor-mediated signal transduction machinery in a cell-free model system | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학교실) | - |
dc.contributor.googleauthor | Cho HI | - |
dc.contributor.googleauthor | Park CG | - |
dc.contributor.googleauthor | Kim J | - |
dc.identifier.doi | 10.1006/abbi.1999.1334 | - |
dc.contributor.localId | A00921 | - |
dc.relation.journalcode | J00211 | - |
dc.identifier.eissn | 1096-0384 | - |
dc.identifier.pmid | 10441372 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0003986199913346 | - |
dc.contributor.alternativeName | Kim, Jong Sun | - |
dc.contributor.affiliatedAuthor | 김종선 | - |
dc.citation.volume | 368 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 221 | - |
dc.citation.endPage | 231 | - |
dc.identifier.bibliographicCitation | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, Vol.368(2) : 221-231, 1999 | - |
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