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베체트병 환자의 혈청에서 인체 진피 미세혈관 내피세포 및 HMEC-1 세포의 표면 조항원을 이용한 항내피세포 항체 검출

Other Titles
 Detection of Antiendothelial Cell Antibodies in Sera of Patients with Behcet 
 이광훈  ;  차명수  ;  방동식  ;  최은선  ;  이성낙 
 Korean Journal of Investigative Dermatology (대한피부연구학회지), Vol.6(1) : 35-45, 1999 
Journal Title
Korean Journal of InvestigativeDermatology(대한피부연구학회지)
Issue Date
Behcet´s disease ; Human dermal microvascular endothelial cell ; Antiendothelial cell antibodies ; HMEC-1 antigen
In this study, we detected circulating antibodies to crude surface antigens isolated from cultured human dermal microvascular endothelial cells(HDMEC) and HMEC-1 in the sera of Beh?et's disease. Also, we evaluated the usefulness of isolated crude surface antigens from HDMEC and HMEC-1 as an antigen for the detection of antiendothelial cell antibody in sera from patients with Beh?et's disease. IgM antibody reacted with the HDMEC surface protein in 63.2% of sera from patients with Beh?et's disease. In systemic lupus erythematosus(SLE), both IgG(52.5%) and IgM antibodies(72.5%) reacted with endothelial cell surface proteins. The same results were obtained in enzyme-linked immunosorbent assay using HMEC-1 surface protein as an antigen. IgM antibody levels were elevated in sera of patients with Beh?et's disease showing reactive circulating immune complex. The percentage of thrombophlebitis was higher in the IgM-positive group compared to the IgM-negative group. Beh?et's disease serum containing anti-endothelial cell IgM antibody reacted with the 44kDa protein of both HDMEC and HMEC-1 in Western blotting. In dot blotting using the 44kDa HDMEC surface protein, IgM-positive sera of SLE patients and normal controls did not react with the protein, but only IgM-positive sera from Beh?et's disease patients reacted with the protein. The results indicate that anti-endothelial cell IgM antibodies reacted with the surface protein isolated from cultured HDMEC in the sera of patients with Beh?et's disease, and these antibodies may be disease-specific antibodies for Beh?et's disease. In addition, these results show that the crude surface antigens isolated from cultured HDMEC and HMEC-1 can be utilized for the detection of antiendothelial cell antibodies in Beh?et's disease.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Bang, Dong Sik(방동식)
Lee, Kwang Hoon(이광훈)
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