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Changes of telomerase and telomere lengths in paired normal and cancer tissues of breast

Authors
 SUN YOUNG RHA  ;  KYU HYUN PARK  ;  TAE SOO KIM  ;  NAE CHOON YOO  ;  WOO ICK YANG  ;  JAE KYUNG ROH  ;  JIN SIK MIN  ;  KYONG SIK LEE  ;  BYUNG SOO KIM  ;  JIN HYUK CHOI  ;  HO YOUNG LIM  ;  HYUN CHEOL CHUNG 
Citation
 International Journal of Oncology, Vol.15(4) : 839-845, 1999 
Journal Title
 International Journal of Oncology 
ISSN
 1019-6439 
Issue Date
1999
MeSH
Adult ; Aged ; Blotting, Southern ; Breast/chemistry* ; Breast/enzymology* ; Breast Neoplasms/enzymology* ; Breast Neoplasms/genetics* ; Dose-Response Relationship, Drug ; Female ; Humans ; Middle Aged ; Polymorphism, Restriction Fragment Length ; Ribonuclease, Pancreatic/pharmacology ; Telomerase/antagonists & inhibitors ; Telomerase/metabolism* ; Telomere/genetics*
Keywords
telomerase ; telomere lengths ; breast cancer
Abstract
To attain the immortal phenotype, cancer cells must overcome the mitotic clock. Telomerase activity has been identified to be activated in malignant tumors including breast cancer. Telomerase activity was evaluated in 71 breast cancer tissues and paired normal tissues with the TRAP (telomerase repeat amplification protocol) assay. Telomerase activity was calculated and translated into arbitrary units by computer-assisted densitometry with the control of telomerase activity in the 293 control cell line. In 59 paired breast tissues with telomerase activity, terminal restriction fragment (TRF) lengths were measured using Southern blotting. Relative inhibition (RI), the ratio of inhibited telomerase activity in each tumor tissue compared to that of the 293 control cell line after pre-treatment with 150 microg/ml of RNAse A, was measured. Sixty-three of 71 cancer tissues showed telomerase activity (88.7%) with 75.3+/-17.9 units in densitometry, while no telomerase activity was detected in their paired normal tissues. Telomerase activity was correlated to node metastasis (p=0.02) and stage (p=0.005), but not to tumor size or the hormonal receptor status. TRF lengths were 11. 0+/-4.7 kb in 59 tumor tissues and 11.7+/-2.2 kb in paired normal tissues. TRF lengths did not correlate to any of the clinical parameters. However changes of TRF lengths in tumor tissues compared to those of normal tissues correlated to telomerase activity. RI in the tumor tissues was proportional to telomerase activity without RNAse A pre-treatment. In breast cancer, telomerase activity was specific to tumor tissues and increased with tumor progression. Telomerase activity and changes in TRF lengths can be used as guidelines in detecting candidates for the telomerase inhibitor.
Full Text
https://www.spandidos-publications.com/ijo/15/4/839
DOI
10.3892/ijo.15.4.839
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Roh, Jae Kyung(노재경)
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Yang, Woo Ick(양우익) ORCID logo https://orcid.org/0000-0002-6084-5019
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/172598
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