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Complete Freund Adjuvant에 의한 피부염증에서 통각과민현상의 기전

Other Titles
 Mechanism of hyperalgesia following cutaneous inflammation by Complete Freund Adjuvant. 
 정용  ;  임중우  ;  정승수  ;  김윤숙  ;  윤덕미  ;  남택상  ;  백광세 
 Korean Journal of Pain (대한통증학회지), Vol.13(2) : 164-174, 2000 
Journal Title
 Korean Journal of Pain (대한통증학회지) 
Issue Date
Complete freund adjuvant ; NMDA receptor ; Nitric oxide ; c-fos
BACKGROUND: After an injury to tissue such as the skin, hyperalgesia develops. Hyperalgesia is characterized by an increase in the magnitude of pain evoked by noxious stimuli. It has been postulated that in the mechanism of hyperalgesia (especially secondary hyperalgesia) and allodynia, a sensitization of central nervous system such as spinal dorsal horn may contribute to development of hyperalgesia. However, the precise mechanism is still unclear. In the present study, we investigated the roles of N-methyl-D-aspartate (NMDA) receptor and nitric oxide (NO) system in the mechanism of hyperalgesia, and their relations with c-fos expression. METHODS: Inflammation was induced by injection of complete Freund adjuvant (CFA) into unilateral hindpaw of Sprague-Dawley rat. Behavioral studies measuring paw withdrawal responses by von Frey filaments and paw withdrawal latencies by radiant heat stimuli and stainings of nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase and c-fos immunoreactivity were performed. The effects of MK-801, an NMDA receptor blocker and N?-nitro-L-arginine (L-NNA), a nitric oxide synthase (NOS) inhibitor were evaluated. RESULTS: 1) Injection of CFA induced mechanical allodynia, mechanical hyperalgesia and thermal hyperalgesia. And it increased the number of NADPH-diaphorase positive neurons and c-fos expression neurons. 2) MK-801 inhibited mechanical hyperalgesia and thermal hyperalgesia induced by CFA and reduced the number of NADPH-diaphorase positive neurons and c-fos expression neurons. 3) L-NNA inhibited the thermal hyperalgesia and reduced the number of NADPH-diaphorase positive neurons, but did not affect the number of c-fos expression neurons. CONCLUSIONS: These results suggest that in the mechanism of mechanical hyperalgesia, NMDA receptor but not NO-system is involved and in the case of thermal hyperalgesia both NMDA receptor and NO system are involved. NO system did not affect the expression of c-fos, but c-fos expression and NOS activity were dependent on the activity of NMDA receptor.
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1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Nam, Taick Sang(남택상)
Leem, Joong Woo(임중우) ORCID logo https://orcid.org/0000-0002-1605-2230
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