배양된 쥐의 골아세포에서 전기장에 의한 Nitric Oxide의 생성

Abstract

Experimental study was conducted to prove the effect of pulsed electromagnetic field (PEMF) on the production of Nitric oxide (NO) from the cultured rat osteoblast-like cells. Calvarium of thirty Sprgue-Dawley rats was digested by sequential collagenase and cultured in-vitro. The osteoblast cell phenotype was confirmed by expression of osteoclacin by immunohistochemistry. PEMF was generated and applied to cultured osteoblast cells. Production of NO was measured by Greiss reaction. Ten minute exposure of PEMF to ostoeblast cell showed increased NO content at 24 and 48 hours(p<0.05). Cultures with different duration of PEMF exposure (10, 20, 30 60 minutes) demonstrated similar responses. In conclusion. this study proved that NO can be generated with PEMF which support the notion that NO can be a possible mediator of PEMF on bone metabolism.