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사람 정상 코점막 및 비용 상피세포에서 점액과 리소자임의 발현 양상.

Other Titles
 Mucin and Lysozyme Expression in the Epithelium of Normal Human Nasal Inferior Turbinate and Polyps 
Authors
 김성식  ;  윤주헌  ;  김문숙  ;  이주형 
Citation
 Korean Journal of Otolaryngology (대한이비인후과학회지), Vol.43(4) : 377-382, 2000 
Journal Title
Korean Journal of Otolaryngology(대한이비인후과학회지)
ISSN
 1225-035X 
Issue Date
2000
Keywords
Mucin ; Lysozyme ; Mucin gene ; Nasal epithelium ; Polyp
Abstract
Background and Objectives:In chronic bronchitis, rhinitis or cystic fibrosis, the number of goblet cells increases along
with hypertrophy of mucous cells in submucosal gland, resulting ineffective mucociliary clearance. But, it is still not fully
understood what role each gene plays in producing airway secretions. This study aimed to figure out which mucin gene is
expressed in the epithelium of normal human nasal mucosa and nasal polyps, and to verify whether the epithelium of nasal
polyp itself contributes to the increased nasal secretion as in chronic sinusitis with nasal polyp. Materials and Methods:
Normal nasal epithelial cells were obtained from the inferior turbinates of ten normal healthy volunteers, and nasal polyps
from six patients who underwent sinus surgery. The epithelial cells were enzymatically isolated, and intracellular levels of
mucin and lysozyme were measured from cell lysates using immunoblot assay. And, RT-PCR was used for the detection of
mucin mRNA and lysozyme mRNA. Results:The level of intracellular mucin was 2.9 times higher in the epithelium of
nasal polyp, and this was statistically significant. Among seven mucin genes (MUC1, 2, 4, 5AC, 5B, 7, 8) expressed in the
epithelium of normal inferior turbinate and polyps, MUC2 and MUC8 were more strongly expressed in the epithelium of
nasal polyp than those of normal inferior turbinate. Conclusion:This results suggest that the polyp epithelium itself is
contributing to increased secretion in chronic sinusitis, and MUC2 and MUC8 are thought to be responsible for this change.
However, further study is required to uncover the full sequence of MUC8 mRNA and its exact function. (Korean J Otolaryngol 2000;43:377-82)
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/172300
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