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성장인자에 의한 토끼의 심부 굴곡건 세포 제1형 교원질의 mRNA 표현 변화

Other Titles
 The Change of Type I Procollagen mRNA Expression by Growth Factors in Rabbit Deep Flexor Tendon Cell 
Authors
 강호정  ;  이우석  ;  신상진  ;  한수봉  ;  강응식 
Citation
 Journal of the Korean Society for Surgery of the Hand, Vol.5(1) : 5-10, 2000 
Journal Title
Journal of the Korean Society for Surgery of the Hand(대한수부외과학회지)
ISSN
 1598-3889 
Issue Date
2000
Keywords
Tendon cell ; growth factor ; proliferation ; type I procollagen mRNA
Abstract
Purpose : This study focuses on the change of the synthesis of type I collagen caused by four kinds of growth factors - epidermal growth factor (EGF), insulin-like growth factor (IGF), platelet-derived growth factor (PDGF), and transforming growth factor (TGF) - known as affecting the metabolism of the flexor tendon in short term culture of cells isolated from rabbit deep flexor tendons. Materials and Methods : Cells were isolated from rabbit deep flexor tendons by enzymatic digestion and cultured with Dulbecco\'s modified eagles medium (DMEM) containing 0.5% fetal bovine serum (FBS). And then four kinds of growth factors (10ng/ml) were given into each. The control cultures were kept as plain cultures without growth factors. On Day 5, Day 10, and Day 15, the cells were collected and cell proliferation rate was carried out by cell counting with hemocytometer. The expression of typeⅠprocollagen mRNA was measured by the Northern blot analysis. Results : In proliferation assay, The 10% FBS group showed the highest cell pro-liferation. The EGF group and the TGF group showed increased cell proliferation compared to the IGF group and PDGF group (p<0.05). The IGF group and PDGF group showed significant increase in type I procollagen mRNA expression (p<0.05), but the EGF group and the TGF group showed no more expression than the control group. Conclusion : Based on this study, we conclude that the PDGF and the IGF to be the most effective growth factors in the early healing process of rabbit flexor tendon.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Ho Jung(강호정) ORCID logo https://orcid.org/0000-0003-0273-1264
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/172201
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