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FCA에 의한 염증모델에서 기계적 이질통에 대한 척수부에서의 NO의 역할.

Other Titles
 The role of spinal NO on the mechanical allodynia in the inflammation model by Freund's Complete Adjuvant. 
Authors
 황재현  ;  김민경  ;  이정찬  ;  최윤  ;  황승준  ;  임중우 
Citation
 Journal of Korean Society of Anesthesiologist (대한마취과학회지), Vol.38(6) : 1068-1074, 2000 
Journal Title
Journal of Korean Society of Anesthesiologist(대한마취과학회지)
ISSN
 0302-5780 
Issue Date
2000
Keywords
Infection ; inflammation ; Freund's complete adjuvant pain: allodynia ; Pharamcology: L-NAME ; methylene blue ; nitric oxide ; sodium nitroprusside
Abstract
BACKGROUND
The effect of spinal nitric oxide (NO) on mechanical allodynia brought about by Freund's complete adjuvant (FCA)-induced inflammation is not known. From our previous experiment nitric oxide synthase (NOS) inhibitor nitroG-L-arginine methyl ester (L-NAME) given intraplantarly during the induction period decreased a mechanical hyperalgesia occurring because of FCA-induced inflammation. Therefore, we investigated the spinal effect of NO on mechanical allodynia after the development of allodynia produced by FCA-induced inflammation in rats.
METHODS
Male Sprague Dawley rats were prepared with lumbar intrathecal catheter implantation. Inflammation was induced in the rats by injecting 0.1 ml of FCA under halothane anesthesia. Behavioral tests were done 1, 3, 6, 24, and 48 hours after injection of FCA. In the other group, intrathecal L-NAME (10 microgram) was given prior to FCA injection to examine the effect of pretreatment. On postinjection day 2, either L-NAME (10 microgram) or methylene blue (10 and 30 microgram) was administered intrathecally after the baseline measurement. The withdrawal response on mechanical allodynia was assessed by applying von Frey filaments to the right lesioned hindpaw and contralateral paw (as control) at 15, 30, 45, 60, 90, and 120 minutes. Sodium nitroprusside was administered intrathecally to determine the reversal effect of increased threshold in the L-NAME group.
RESULTS
Injection of FCA produced a significant mechanical allodynia over time. Pretreatment with L-NAME did not prevent such a mechanical allodynia. Intrathecal L-NAME, but not methylene blue, reduced the mechanical allodynia, which was reversed by sodium nitroprusside.
Files in This Item:
T200003077.pdf Download
DOI
10.4097/kjae.2000.38.6.1068
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Leem, Joong Woo(임중우) ORCID logo https://orcid.org/0000-0002-1605-2230
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/172070
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