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원발성 소세포폐암에서 염색체 5번의 장완에 위치한 종양억제유전자좌의 확인

Other Titles
 Identification of Tumor Supressor Loci on the Long Arm of Chromosome 5 in Primary Small Cell Lung Cancers 
Authors
 조은송  ;  김호근  ;  조철호  ;  장 준  ;  정경영  ;  김영삼  ;  박재민  ;  김성규  ;  김세규 
Citation
 Tuberculosis and Respiratory Diseases (결핵 및 호흡기질환), Vol.49(1) : 49-59, 2000 
Journal Title
TUBERCULOSIS AND RESPIRATORY DISEASES(결핵 및 호흡기질환)
ISSN
 0378-006 
Issue Date
2000
MeSH
Arm* ; Carcinogenesis ; Chromosomes, Human, Pair 5* ; Clone Cells ; Cloning, Organism ; Cytogenetics ; DNA ; Genes, Tumor Suppressor ; Lung ; Neoplasms* ; Lung* ; Microsatellite Repeats ; Small Cell Lung Carcinoma
Keywords
Small cell lung cancer ; Chromosome 5 ; LOH ; Tumor suppressor
Abstract
Background

Recent cytogenetic studies indicated that loss of the long arm of chromosome 5 is a frequent event in small cell lung cancer (SCLC), suggesting the presence of a tumor suppressor gene is its place. To map the precise tumor-suppressor loci on the chromosome arm for further positional cloning efforts, we tested 15 primary SCLCs.


Methods

The DNAs extracted from paraffin-embedded tissue blocks with primary tumor and corresponding control tissue were investigated. Nineteen polymorphic microsatellite markers located in the long arm of chromosome 5 were used in the microsatellite analysis.


Results

We found that ten (66.7%) of 15 tumors exhibited LOH in at least one of tested microsatellite markers. Two (13%) of 10 tumors exhibiting LOH lost a larger area in chromosome 5q. LOH was observed in five common deleted regions at 5q. Among those areas, LOH between 5q34-qter and 5q35.2-35.3 was most frequent (75%). LOH was also observed in more than 50% of the tumors at four at other regions, between 5q14-15 and 5q23-31, 5q31.1, 5q31.3-33.3, and 5q34-35. Three of 15 tumors exhibited shifted bands in at least one of the tested microsatellite markers. Shifted bands occurred in 2.5% (7 of 285) of the loci tested.


Conclusion

Our data demonstrated that at least five tumor-suppressor loci exist in the long arm of chromosome 5 and that they may play an important role in small cell lung cancer tumorigenesis.
Files in This Item:
T200002637.pdf Download
DOI
10.4046/trd.2000.49.1.49
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Kyu(김세규)
Kim, Young Sam(김영삼) ORCID logo https://orcid.org/0000-0001-9656-8482
Kim, Hogeun(김호근)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171967
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