121 178

Cited 4 times in

Influence of the sequence variation of the HLA-DR promoters derived from human melanoma cell lines on nuclear protein binding and promoter activity

DC Field Value Language
dc.contributor.author박전한-
dc.date.accessioned2019-11-11T05:28:29Z-
dc.date.available2019-11-11T05:28:29Z-
dc.date.issued2000-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/171908-
dc.description.abstractIn previous studies we reported that the expression of HLA-DR on melanoma cell lines was differentially modulated by IFN- gamma and that the transcription rate was responsible for this differential modulation. We have also reported the nucleotide sequence variations in the promoter region of HLA-DR genes, and proposed that differences in the promoter activity by the sequence variations of the HLA-DR promoters might contribute to such a differential transcriptional regulation at the promoter level. In this study, in order to assess whether the sequence variations of the HLA-DR promoters affect the factor binding and exert influence on the promoter activity, nuclear factor binding to our previous six HLA-DRA and fourteen HLA-DRB promoter clones was evaluated with the nuclear protein extracted from a B-lymphoblastoid cell line (BLCL), BH, together with the chloramphenicol acetyltransferase (CAT) reporter assay. In the HLA-DRA promoters, clone #35 containing one bp nucleotide sequence variation at the octamer binding site (OCT) (GATTTGC to GATCTGC) showed relatively weak factor binding. In the HLA-DRB promoters, clusters I, III, and IV of our previous HLA-DRB promoter homologues, containing one bp nucleotide sequence variation (GATTCG) in their Y boxes exhibited weak factor binding and CAT activity compared to other clusters (GATTGG) that showed strong factor binding and CAT activity. This data suggests chat the binding patterns of transcription factors influenced by the nucleotide sequence variations of the HLA-DR promoter could affect the promoter activity and the DNA sequence elements in the HLA-DR promoter could mediate transcriptional regulation.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYonsei Medical Journal-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHBase Sequence/genetics-
dc.subject.MESHGenetic Variation*-
dc.subject.MESHHLA-DR Antigens/genetics*-
dc.subject.MESHHumans-
dc.subject.MESHMelanoma/genetics*-
dc.subject.MESHMelanoma/pathology-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHNuclear Proteins/metabolism*-
dc.subject.MESHPromoter Regions, Genetic/genetics*-
dc.subject.MESHPromoter Regions, Genetic/physiology*-
dc.subject.MESHTumor Cells, Cultured-
dc.titleInfluence of the sequence variation of the HLA-DR promoters derived from human melanoma cell lines on nuclear protein binding and promoter activity-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학교실)-
dc.contributor.googleauthorTae Jin Lee-
dc.contributor.googleauthorJong Kim-
dc.contributor.googleauthorJeon Han Park-
dc.identifier.doi10.3349/ymj.2000.41.5.593-
dc.contributor.localIdA01641-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid11079619-
dc.subject.keywordHLA-DR promoter-
dc.subject.keywordsequence variations-
dc.subject.keywordfactor binding-
dc.subject.keywordpromoter activity-
dc.subject.keywordtranscriptional regulation-
dc.contributor.alternativeNamePark, Jeon Han-
dc.contributor.affiliatedAuthor박전한-
dc.citation.volume41-
dc.citation.number5-
dc.citation.startPage593-
dc.citation.endPage599-
dc.identifier.bibliographicCitationYonsei Medical Journal, Vol.41(5) : 593-599, 2000-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.