Cited 27 times in
Chlorophyllin suppression of lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박광균 | - |
dc.date.accessioned | 2019-11-11T05:25:46Z | - |
dc.date.available | 2019-11-11T05:25:46Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0041-008X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/171869 | - |
dc.description.abstract | Chlorophyllin (CHL), a water-soluble derivative of chlorophyll, functions as an anticarcinogen and antioxidant. In the present study, we investigated the effect of CHL on nitric oxide production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Treatment with CHL inhibited nitric oxide production in the LPS-stimulated RAW 264. 7 cells in a dose-related manner. Competitive RT-PCR analysis, using a DNA competitor as an internal standard, demonstrated that the treatment with 1, 10, and 50 microM CHL decreased LPS-induced iNOS mRNA expression in a concentration-dependent manner. Since the expression of the iNOS gene is mainly regulated by NF-kappaB, we then examined the effects of CHL on the NF-kappaB DNA binding activity, using an electrophoretic mobility shift assay. CHL down-regulated the NF-kappaB DNA binding on its cognate recognition site at the concentrations just noted. Employing a transfection and reporter gene expression system with p(NF-kappaB)(3)-chloramphenicol acetyl transferase (CAT), the treatment of CHL produced a dose-dependent inhibition of CAT activity in RAW 264.7 cells. Furthermore, CHL partially restored LPS-decreased IkappaBalpha, an inhibitory protein against NF-kappaB activation, in the cytosolic extract from the LPS-treated cells determined by immunoblot analysis. CHL also protected the hydroxyl radical-induced cytotoxicity in RAW 264.7 cells, indicating its antioxidant effect. These results suggest that CHL suppresses the nitric oxide production and iNOS mRNA expression mediated by the inhibition of NF-kappaB activation, and its action mechanism may be based on its antioxidant effect. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Academic Press | - |
dc.relation.isPartOf | Toxicology and Applied Pharmacology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antimutagenic Agents/pharmacology* | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Nucleus/metabolism | - |
dc.subject.MESH | Chloramphenicol O-Acetyltransferase/genetics | - |
dc.subject.MESH | Chlorophyllides/pharmacology* | - |
dc.subject.MESH | DNA/metabolism | - |
dc.subject.MESH | DNA-Binding Proteins/drug effects | - |
dc.subject.MESH | DNA-Binding Proteins/metabolism | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Electrophoresis | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology* | - |
dc.subject.MESH | Genes, Reporter | - |
dc.subject.MESH | Lipopolysaccharides/pharmacology | - |
dc.subject.MESH | Lymphocyte Activation | - |
dc.subject.MESH | Macrophages/drug effects* | - |
dc.subject.MESH | Macrophages/enzymology | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | NF-kappa B/genetics | - |
dc.subject.MESH | NF-kappa B/metabolism | - |
dc.subject.MESH | Nitric Oxide Synthase/antagonists & inhibitors* | - |
dc.subject.MESH | Nitric Oxide Synthase/genetics | - |
dc.subject.MESH | Nitric Oxide Synthase/metabolism | - |
dc.subject.MESH | Nitric Oxide Synthase Type II | - |
dc.subject.MESH | Nitrites/metabolism | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Salmonella typhimurium | - |
dc.subject.MESH | Transfection | - |
dc.title | Chlorophyllin suppression of lipopolysaccharide-induced nitric oxide production in RAW 264.7 cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학교실) | - |
dc.contributor.googleauthor | Kyung-Joo Cho | - |
dc.contributor.googleauthor | Seung Hyun Han | - |
dc.contributor.googleauthor | Bu Yeo Kim | - |
dc.contributor.googleauthor | Seong-Gu Hwang | - |
dc.contributor.googleauthor | Kwang-Kyun Park | - |
dc.contributor.googleauthor | Kyu-Hwan Yang | - |
dc.contributor.googleauthor | An-Sik Chung | - |
dc.identifier.doi | 10.1006/taap.2000.8958 | - |
dc.contributor.localId | A01429 | - |
dc.relation.journalcode | J02742 | - |
dc.identifier.eissn | 1096-0333 | - |
dc.identifier.pmid | 10896853 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0041008X00989580 | - |
dc.subject.keyword | chlorophyllin | - |
dc.subject.keyword | nitric oxide | - |
dc.subject.keyword | lipopolysaccharide | - |
dc.subject.keyword | NF-κB | - |
dc.contributor.alternativeName | Park, Kwang Kyun | - |
dc.contributor.affiliatedAuthor | 박광균 | - |
dc.citation.volume | 166 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 120 | - |
dc.citation.endPage | 127 | - |
dc.identifier.bibliographicCitation | Toxicology and Applied Pharmacology, Vol.166(2) : 120-127, 2000 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.