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Isozyme electrophoresis patterns of the liver fluke, Clonorchis sinensis from Kimhae, Korea and from Shenyang, China

Authors
 Gab-Man Park  ;  Tai-Soon Yong  ;  Kyung-il Im  ;  Kyu Je Lee 
Citation
 Korean Journal of Parasitology, Vol.38(1) : 45-48, 2000 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
2000
MeSH
Aconitate Hydratase/genetics ; Aconitate Hydratase/isolation & purification ; Alleles ; Animals ; China ; Clonorchis sinensis/enzymology* ; Clonorchis sinensis/genetics ; Electrophoresis, Starch Gel ; Esterases/genetics ; Esterases/isolation & purification ; Glucosephosphate Dehydrogenase/genetics ; Glucosephosphate Dehydrogenase/isolation & purification ; Isoenzymes/genetics ; Isoenzymes/isolation & purification ; Korea
Keywords
Clonorchis sinensis ; isozyme ; electrophoresis ; genetic identity ; China ; Korea
Abstract
An enzyme analysis of the liver fluke, Clonorchis sinensis from Kimhae, Korea and from Shenyang, China was conducted using a horizontal starch gel electrophoresis in order to elucidate their genetic relationships. A total of eight enzymes was employed from two different kinds of buffer systems. Two loci from each enzyme of aconitase and esterase (alpha-Na and beta-Na); and only one locus each from six enzymes, glucose-6-phosphate dehydrogenase (G6PD), alpha-glycerophosphate dehydrogenase (GPD), 3-hydroxybutyrate dehydrogenase (HBDH), malate dehydrogenase (MDH), phosphoglucose isomerase (PGI), and phosphoglucomutase (PGM) were detected. Most of loci in two populations of C. sinensis showed homozygous monomorphic banding patterns and one of them, GPD was specific as genetic markers between two different populations. However, esterase (alpha-Na), GPD, HBDH and PGI loci showed polymorphic banding patterns. Two populations of C. sinensis were more closely clustered within the range of genetic identity value of 0.998-1.0. In summarizing the above results, two populations of C. sinensis employed in this study showed mostly monomorphic enzyme protein banding patterns, and genetic differences specific between two populations.
Files in This Item:
T200001672.pdf Download
DOI
10.3347/kjp.2000.38.1.45
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171780
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