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Gentamicin induced apoptosis of renal tubular epithelial (LLC-PK1) cells

 Kyu Hun Choi  ;  Tae Il Kim  ;  Deug Lim Chong  ;  Ho Yung Lee  ;  Dae Suk Han 
 Korean Journal of Internal Medicine, Vol.15(3) : 218-223, 2000 
Journal Title
 Korean Journal of Internal Medicine 
Issue Date
Animals ; Anti-Bacterial Agents/toxicity* ; Apoptosis/drug effects* ; Gentamicins/toxicity* ; Kidney Tubules, Proximal/drug effects* ; LLC-PK1 Cells ; Swine ; fas Receptor/analysis
Gentamicins ; LLC-PK, cells ; Apoptosis
Nephrotoxicity is a major limiting factor in the use of aminoglycoside antibiotics, the mechanisms for which are still speculative. To clarify the mechanisms of renal tubular cell death induced by aminoglycosides, we examined the renal proximal tubule-like cell line, LLC-PK1, after inducing apoptosis through a chronic treatment with gentamicin (GM). Changes in the expression of the Fas were also investigated. On flow cytometric analysis, 5.7 +/- 3.3% of the control cells appeared in a region of decreased forward light scatter and increased side light scatter, where both indices represent the characteristics of apoptotic cell death. Compared to the control, treatment with 10 mM of GM for 15 days significantly increased the proportion of cells in the apoptotic region to 23.9 +/- 8.5%. This finding was supported by electrophoretic analysis of the DNA extracted from the GM-treated cells, where a series of bands corresponding to integer multiples of 180 to 200 base pairs was visualized. However, the 15-day GM treatment did not cause a significant elevation in the expression of the 45 kD Fas protein, the cell surface molecule that stimulates apoptosis, by Western blot analysis. In conclusion, long-term exposure to GM induces apoptosis of the renal tubular epithelial cells, and this process may contribute to some of the aminoglycoside nephrotoxicities. Further studies are needed on the mechanism(s) of apoptosis induced by GM.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Choi, Kyu Hun(최규헌) ORCID logo https://orcid.org/0000-0003-0095-9011
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