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Localization of a major allergen, Der p 2, in the gut and faecal pellets of Dermatophagoides pteronyssinus

Authors
 GM Park  ;  SM Lee  ;  IY Lee  ;  HI Ree  ;  KS Kim  ;  CS Hong  ;  TS Yong 
Citation
 Clinical and Experimental Allergy, Vol.30(9) : 1293-1297, 2000 
Journal Title
 Clinical and Experimental Allergy 
ISSN
 0954-7894 
Issue Date
2000
MeSH
Allergens/analysis* ; Animals ; Antibodies, Monoclonal/immunology ; Antigens, Dermatophagoides ; Digestive System/immunology ; Feces/chemistry* ; Female ; Fluorescent Antibody Technique ; Glycoproteins/analysis* ; Glycoproteins/immunology ; Male ; Mites/immunology* ; Mites/ultrastructure
Keywords
house dust mite ; Dermatophagoides pteronyssinus ; allergen localization ; Der p 2
Abstract
BACKGROUND: The house dust mite Dermatophagoides ptronyssinus is one of the most significant indoor sensitizing agents of allergy. Allergen localization may indicate the importance of secreted materials, faeces, and nonexcreted mite body components as allergen sources. OBJECTIVE: This study attempted to localize the sites and concentrations of Der p 2 in the cryostat sections of D. pteronyssinus using antirecombinant Der p 2 monoclonal antibody. METHODS: Male and female mites and mite faeces collected separately from both sexes were used. Live mites were embedded and serial cryostat sections for light microscopy were performed. Anti-recombinant Der p 2 monoclonal antibody previously produced by the authors was used. For immunoprobing, mite cryostat sections were incubated in the following antibody-containing solutions: monoclonal antibody against Der p 2 was initially applied to the sections and fluorescent isothiocyanate conjugated antimouse immunoglobulin G was reacted as the secondary antibody. The faecal pellets were treated the same as described above. RESULTS: Immunofluorescent probing of cryostat sections with the monoclonal antibody showed labelling of the gut lining, gut contents and defecated faecal pellets. No other internal organs were identified as positively labelled. CONCLUSION: This study suggested that a major allergen, Der p 2, found in the house dust mite D. pteronyssinus is derived from the digestive tract and concentrated in the faeces.
Full Text
https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1365-2222.2000.00883.x
DOI
10.1046/j.1365-2222.2000.00883.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Hong, Chein Soo(홍천수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/171750
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